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dc.contributor.authorBianchet, Chantal
dc.contributor.authorWong, Aloysius Tze
dc.contributor.authorQuaglia, Mara
dc.contributor.authorAlquraishi, May Majed
dc.contributor.authorGehring, Christoph A
dc.contributor.authorNtoukakis, Vardis
dc.contributor.authorPasqualini, Stefania
dc.date.accessioned2018-11-21T13:10:17Z
dc.date.available2018-11-21T13:10:17Z
dc.date.issued2018-11-03
dc.identifier.citationBianchet C, Wong A, Quaglia M, Alqurashi M, Gehring C, et al. (2018) An Arabidopsis thaliana leucine-rich repeat protein harbors an adenylyl cyclase catalytic center and affects responses to pathogens. Journal of Plant Physiology. Available: http://dx.doi.org/10.1016/j.jplph.2018.10.025.
dc.identifier.issn0176-1617
dc.identifier.doi10.1016/j.jplph.2018.10.025
dc.identifier.urihttp://hdl.handle.net/10754/629936
dc.description.abstractAdenylyl cyclases (ACs) catalyze the formation of the second messenger cAMP from ATP. Here we report the characterization of an Arabidopsis thaliana leucine-rich repeat (LRR) protein (At3g14460; AtLRRAC1) as an adenylyl cyclase. Using an AC-specific search motif supported by computational assessments of protein models we identify an AC catalytic center within the N-terminus and demonstrate that AtLRRAC1 can generate cAMP in vitro. Knock-out mutants of AtLRRAC1 have compromised immune responses to the biotrophic fungus Golovinomyces orontii and the hemibiotrophic bacteria Pseudomonas syringae, but not against the necrotrophic fungus Botrytis cinerea. These findings are consistent with a role of cAMP-dependent pathways in the defense against biotrophic and hemibiotrophic plant pathogens.
dc.description.sponsorshipThis project was funded by King Abdullah University of Science and Technology, Royal Society, Biotechnology and Biological Science Research Council grants (BB/L019345/1 and BB/M017982/1) and “Fondo di Ateneo per la Ricerca di Base 2015” financed by University of Perugia. We are grateful to Aleš Lebeda and Božena Sedláková (Palacký University, Olomouc) for providing the Golovinomyces orontii isolates. AW is supported by grants from the National Natural Science Foundation of China (31850410470) and the Zhejiang Provincial Natural Science Foundation of China (Q19C130001).
dc.publisherElsevier BV
dc.relation.urlhttps://www.sciencedirect.com/science/article/pii/S0176161718305650
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Journal of Plant Physiology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Plant Physiology, [, , (2018-11-03)] DOI: 10.1016/j.jplph.2018.10.025 . © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectcAMP
dc.subjectAdenylyl cyclase
dc.subjectArabidopsis thaliana
dc.subjectBotrytis cinerea
dc.subjectGolovinomycesorontii
dc.subjectPseudomonas syringae pv. tomato
dc.titleAn Arabidopsis thaliana leucine-rich repeat protein harbors an adenylyl cyclase catalytic center and affects responses to pathogens
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentChemical Engineering Program
dc.contributor.departmentMolecular Signalling Group
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalJournal of Plant Physiology
dc.eprint.versionPost-print
dc.contributor.institutionDepartment of Chemistry, Biology and Biotechnology, University of Perugia, Borgo XX giugno, 74, 06121 Perugia, Italy
dc.contributor.institutionCollege of Science and Technology, Wenzhou-Kean University, 88 Daxue Road, Ouhai, Wenzhou, Zhejiang Province, 325060, China
dc.contributor.institutionDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, Borgo XX giugno, 74, 06121 Perugia, Italy
dc.contributor.institutionWarwick Integrative Synthetic Biology Centre, The University of Warwick, Coventry, CV4 7AL, UK
dc.contributor.institutionSchool of Life Sciences, University of Warwick, CV4 7AL, Coventry, UK
kaust.personAlquraishi, May Majed
kaust.personGehring, Christoph A.
refterms.dateFOA2018-11-22T11:28:53Z
dc.date.published-online2018-11-03
dc.date.published-print2019-01


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