An Arabidopsis thaliana leucine-rich repeat protein harbors an adenylyl cyclase catalytic center and affects responses to pathogens
Wong, Aloysius Tze
Alquraishi, May Majed
Gehring, Christoph A
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Chemical Engineering Program
Molecular Signalling Group
Physical Science and Engineering (PSE) Division
Online Publication Date2018-11-03
Print Publication Date2019-01
Permanent link to this recordhttp://hdl.handle.net/10754/629936
MetadataShow full item record
AbstractAdenylyl cyclases (ACs) catalyze the formation of the second messenger cAMP from ATP. Here we report the characterization of an Arabidopsis thaliana leucine-rich repeat (LRR) protein (At3g14460; AtLRRAC1) as an adenylyl cyclase. Using an AC-specific search motif supported by computational assessments of protein models we identify an AC catalytic center within the N-terminus and demonstrate that AtLRRAC1 can generate cAMP in vitro. Knock-out mutants of AtLRRAC1 have compromised immune responses to the biotrophic fungus Golovinomyces orontii and the hemibiotrophic bacteria Pseudomonas syringae, but not against the necrotrophic fungus Botrytis cinerea. These findings are consistent with a role of cAMP-dependent pathways in the defense against biotrophic and hemibiotrophic plant pathogens.
CitationBianchet C, Wong A, Quaglia M, Alqurashi M, Gehring C, et al. (2018) An Arabidopsis thaliana leucine-rich repeat protein harbors an adenylyl cyclase catalytic center and affects responses to pathogens. Journal of Plant Physiology. Available: http://dx.doi.org/10.1016/j.jplph.2018.10.025.
SponsorsThis project was funded by King Abdullah University of Science and Technology, Royal Society, Biotechnology and Biological Science Research Council grants (BB/L019345/1 and BB/M017982/1) and “Fondo di Ateneo per la Ricerca di Base 2015” financed by University of Perugia. We are grateful to Aleš Lebeda and Božena Sedláková (Palacký University, Olomouc) for providing the Golovinomyces orontii isolates. AW is supported by grants from the National Natural Science Foundation of China (31850410470) and the Zhejiang Provincial Natural Science Foundation of China (Q19C130001).
JournalJournal of Plant Physiology