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    Theileria\n highjacks JNK2 into a complex with the macroschizont GPI-anchored surface protein p104

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    Lat-_et_al-2018-Cellular_Microbiology.pdf
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    Type
    Article
    Authors
    de Laté, Perle Latré
    Haidar, Malak
    Ansari, Hifzur Rahman cc
    Tajeri, Shahin
    Szarka, Eszter
    Alexa, Anita
    Woods, Kerry cc
    Reményi, Attila
    Pain, Arnab cc
    Langsley, Gordon cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Computational Bioscience Research Center (CBRC)
    Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
    Bioscience Program
    Pathogen Genomics Laboratory
    KAUST Grant Number
    URF/1/2610-01-01
    BAS/1/1020-01-01
    URF/1/2610-01-01
    Date
    2018-12-05
    Permanent link to this record
    http://hdl.handle.net/10754/629855
    
    Metadata
    Show full item record
    Abstract
    Constitutive JNK activity characterizes bovine T and B cells infected with Theileria parva, and B cells and macrophages infected with T. annulata. Here, we show that T. annulata infection of macrophages manipulates JNK activation by recruiting JNK2 and not JNK1 to the parasite surface, whereas JNK1 is found predominantly in the host cell nucleus. At the parasite's surface JNK2 forms a complex with p104 a GPI-anchored T. annulata plasma membrane protein. Sequestration of JNK2 depended on PKA-mediated phosphorylation of a JNK-binding motif common to T. parva and a cell penetrating peptide harbouring the conserved p104 JNK-binding motif competitively ablated binding, whereupon liberated JNK2 became ubiquitinated and degraded. Cytosolic sequestration of JNK2 suppressed small mitochondrial ARF-mediated autophagy, whereas it sustained nuclear JNK1 levels, c-Jun phosphorylation and matrigel traversal. Therefore, T. annulata sequestration of JNK2 contributes to both survival and dissemination of Theileria-transformed macrophages.
    Citation
    De Laté PL, Haidar M, Ansari H, Tajeri S, Szarka E, et al. (2018) Theileria\n highjacks JNK2 into a complex with the macroschizont GPI-anchored surface protein p104. Cellular Microbiology: e12973. Available: http://dx.doi.org/10.1111/cmi.12973.
    Sponsors
    We would like to thank Professor Brian Shiels for gift of antibodies to Theileria p104 (mAb 1C12). This work was supported by the grant Labex ParaFrap [ANR-11-LABX-0024] and core funding from INSERM and the CNRS awarded to GL and a CRG4 grant [URF/1/2610-01-01] from the Office for Sponsored Research (OSR) in King Abdullah University of Science and Technology (KAUST) award to AP and GL and the faculty baseline fund (BAS/1/1020-01-01) awarded to AP. AR acknowledges a Hungarian NKFIH grant NN114309. KW ackowledges Swiss National Science Foundation (SNF) Grant number PZ00P3_154689. PLdL and ST were recipients of ParaFrap post-doctoral fellowships and MH and HRH were supported by the URF/1/2610-01-01 grant from KAUST. There are no competing financial interests in relation to the work described.
    Publisher
    Wiley
    Journal
    Cellular Microbiology
    DOI
    10.1111/cmi.12973
    Additional Links
    https://onlinelibrary.wiley.com/doi/abs/10.1111/cmi.12973
    ae974a485f413a2113503eed53cd6c53
    10.1111/cmi.12973
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

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