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dc.contributor.authorBettgenhaeuser, Jan
dc.contributor.authorGardiner, Matthew
dc.contributor.authorSpanner, Rebecca
dc.contributor.authorGreen, Phon
dc.contributor.authorHernández-Pinzón, Inmaculada
dc.contributor.authorHubbard, Amelia
dc.contributor.authorAyliffe, Michael
dc.contributor.authorMoscou, Matthew J.
dc.date.accessioned2018-10-07T08:45:43Z
dc.date.available2018-10-07T08:45:43Z
dc.date.issued2018-09-28
dc.identifier.citationBettgenhaeuser J, Gardiner M, Spanner R, Green P, Hernández-Pinzón I, et al. (2018) The genetic architecture of colonization resistance in Brachypodium distachyon to non-adapted stripe rust (Puccinia striiformis) isolates. PLOS Genetics 14: e1007637. Available: http://dx.doi.org/10.1371/journal.pgen.1007637.
dc.identifier.issn1553-7404
dc.identifier.doi10.1371/journal.pgen.1007637
dc.identifier.urihttp://hdl.handle.net/10754/628886
dc.description.abstractMultilayered defense responses ensure that plants are hosts to only a few adapted pathogens in the environment. The host range of a plant pathogen depends on its ability to fully overcome plant defense barriers, with failure at any single step sufficient to prevent life cycle completion of the pathogen. Puccinia striiformis, the causal agent of stripe rust (=yellow rust), is an agronomically important obligate biotrophic fungal pathogen of wheat and barley. It is generally unable to complete its life cycle on the non-adapted wild grass species Brachypodium distachyon, but natural variation exists for the degree of hyphal colonization by Puccinia striiformis. Using three B. distachyon mapping populations, we identified genetic loci conferring colonization resistance to wheat-adapted and barley-adapted isolates of P. striiformis. We observed a genetic architecture composed of two major effect QTLs (Yrr1 and Yrr3) restricting the colonization of P. striiformis. Isolate specificity was observed for Yrr1, whereas Yrr3 was effective against all tested P. striiformis isolates. Plant immune receptors of the nucleotide binding, leucine-rich repeat (NB-LRR) encoding gene family are present at the Yrr3 locus, whereas genes of this family were not identified at the Yrr1 locus. While it has been proposed that resistance to adapted and non-adapted pathogens are inherently different, the observation of (1) a simple genetic architecture of colonization resistance, (2) isolate specificity of major and minor effect QTLs, and (3) NB-LRR encoding genes at the Yrr3 locus suggest that factors associated with resistance to adapted pathogens are also critical for non-adapted pathogens.
dc.description.sponsorshipWe thank John H. Doonan for access to the ABR6 x Bd21 population prior to publication, John P. Vogel for advance access to the Joint Genome Institute resequencing data, Pilar Catalán and Luis A. J. Mur for providing seed, Rosemary Bayles, Eric R. Ward, and Jonathan D. G. Jones for early discussions on the experimental design, Diane G. O. Saunders for discussions on the P. striiformis phylogeny, Brande B. H. Wulff and Paul Nicholson for helpful feedback and comments on the manuscript, Sue Banfield and the John Innes Centre Horticultural Service for assistance with plant growth, and Katie O’Neil for assistance with the Luc1 x Jer1 genetic map. KASP genotyping was performed at the John Innes Centre genotyping facility.
dc.publisherPublic Library of Science (PLoS)
dc.relation.urlhttps://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007637
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleThe genetic architecture of colonization resistance in Brachypodium distachyon to non-adapted stripe rust (Puccinia striiformis) isolates
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalPLOS Genetics
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionThe Sainsbury Laboratory, Norwich, United Kingdom
dc.contributor.institutionNational Institute of Agricultural Botany, Cambridge, United Kingdom
dc.contributor.institutionCommonwealth Scientific and Industrial Research Organisation, Agriculture and Food, Canberra, Australian Capital Territory, Australia
dc.contributor.institutionSchool of Biological Sciences, University of East Anglia, Norwich, United Kingdom
kaust.personBettgenhaeuser, Jan
refterms.dateFOA2018-10-07T10:51:33Z


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This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.