dc.contributor.author Alsharif, Nouf dc.contributor.author Martiinez-Banderas, Aldo I. dc.contributor.author Merzaban, Jasmeen dc.contributor.author Ravasi, Timothy dc.contributor.author Kosel, Jürgen dc.date.accessioned 2018-09-27T07:13:10Z dc.date.available 2018-09-27T07:13:10Z dc.date.issued 2018-09-21 dc.identifier.citation Alsharif NA, Martiinez-Banderas AI, Merzaban J, Ravasi T, Kosel J (2018) Biofunctionalizing Magnetic Nanowires Toward Targeting and Killing Leukemia Cancer Cells. IEEE Transactions on Magnetics: 1–5. Available: http://dx.doi.org/10.1109/TMAG.2018.2864245. dc.identifier.issn 0018-9464 dc.identifier.issn 1941-0069 dc.identifier.doi 10.1109/TMAG.2018.2864245 dc.identifier.uri http://hdl.handle.net/10754/628818 dc.description.abstract The objective of targeting technologies in the field of nanomedicine is to specifically focus therapies on diseased cells in order to deliver the treatment to these cells without harming healthy ones. The use of magnetic materials in such biomedical applications offers many advantages. They can be functionalized with an agent that enhances the biocompatibility and the targeting while, at the same time, they can be controlled and monitored remotely. Iron nanowires (Fe NWs), which usually have a native oxide shell, are specifically attractive, since they are highly biocompatible, strongly magnetic, and can be coated with different biological agents. Shape anisotropy makes these NWs permanently magnetic and, therefore, can be exploited for multifaceted remote manipulations, rendering them versatile nano-robots. As such, they have been used before in combination with a magnetic field to induce cancer cell death. In order to minimize the side effects of this method, this paper aims to enhance the targeting ability of these NWs toward particular cells. Specifically, leukemic cells are targeted by functionalizing Fe NWs with anti-CD44 antibodies, a cell surface marker, which is overexpressed in leukemic cells compared to healthy blood cells. Fe NWs were electrochemically fabricated with an average diameter of 35 nm and a length around $3 \mu$m. They were coated with bovine serum albumin to facilitate their conjugation covalently with an anti-CD44 antibody by using 3-(3-dimethyl-aminopropyl) carbodiimide and of N-hydroxysuccinimide. In order to confirm the presence of anti-CD44 antibodies on the surface of the NWs, immunostaining and Fourier transform infrared spectroscopy were used. In addition, cytotoxicity effects of bare Fe NWs and coated and functionalized NWs were studied by using cell proliferation assays. These studies illustrated that the NWs have a high level of biocompatibility. dc.description.sponsorship This work was supported by the King Abdullah University of Science and Technology. dc.publisher Institute of Electrical and Electronics Engineers (IEEE) dc.relation.url https://ieeexplore.ieee.org/document/8457484/ dc.rights (c) 2018 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other users, including reprinting/ republishing this material for advertising or promotional purposes, creating new collective works for resale or redistribution to servers or lists, or reuse of any copyrighted components of this work in other works. Released under the IEEE Open Access Publishing Agreement. dc.subject Antibodies dc.subject Cancer dc.subject Cells (biology) dc.subject Coatings dc.subject functionalization dc.subject Iron dc.subject iron dc.subject Magnetic anisotropy dc.subject Nanowires dc.subject nanowires (NWs) dc.subject Surface treatment dc.title Biofunctionalizing Magnetic Nanowires Toward Targeting and Killing Leukemia Cancer Cells dc.type Article dc.contributor.department Biological and Environmental Sciences and Engineering (BESE) Division dc.contributor.department Bioscience Program dc.contributor.department Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division dc.contributor.department Electrical Engineering Program dc.identifier.journal IEEE Transactions on Magnetics dc.eprint.version Post-print kaust.person Alsharif, Nouf Adnan kaust.person Martiinez-Banderas, Aldo I. kaust.person Merzaban, Jasmeen S. kaust.person Ravasi, Timothy kaust.person Kosel, Jürgen refterms.dateFOA 2018-09-27T11:21:16Z dc.date.published-online 2018-09-21 dc.date.published-print 2019-02
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