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dc.contributor.authorAbuauf, Haneen W.
dc.contributor.authorHaider, Imran
dc.contributor.authorJia, Kunpeng
dc.contributor.authorAblazov, Abdugaffor
dc.contributor.authorMi, Jianing
dc.contributor.authorBlilou, Ikram
dc.contributor.authorAl-Babili, Salim
dc.date.accessioned2018-09-16T12:40:50Z
dc.date.available2018-09-16T12:40:50Z
dc.date.issued2018-09-11
dc.identifier.citationAbuauf H, Haider I, Jia K-P, Ablazov A, Mi J, et al. (2018) The Arabidopsis DWARF27 gene encodes an all-trans-/9-cis-β-carotene isomerase and is induced by auxin, abscisic acid and phosphate deficiency. Plant Science. Available: http://dx.doi.org/10.1016/j.plantsci.2018.06.024.
dc.identifier.issn0168-9452
dc.identifier.doi10.1016/j.plantsci.2018.06.024
dc.identifier.urihttp://hdl.handle.net/10754/628729
dc.description.abstractStrigolactones (SLs) are carotenoid-derived plant hormones that influence various aspects of plant growth and development in response to environmental conditions, especially nutrients deficiency. SLs are synthesized via a strict stereo-specific core pathway that leads to the intermediate carlactone, requiring the iron-containing polypeptide DWARF27 (D27) and the carotenoid cleavage dioxygenases 7 (CCD7) and 8 (CCD8). It has been shown that the rice OsD27 is a β-carotene isomerase catalyzing the interconversion of all-trans- into 9-cis-β -carotene. However, data about the enzymatic activity of D27 from other species are missing. Here, we investigated the activity and substrate specificity of the Arabidopsis AtD27 by testing a broad range of carotenoid substrates. Both in vivo and in vitro assays show that AtD27 catalyzes the reverse isomerization of all-trans-/9-cis-β-carotene. AtD27 did not isomerize 13-cis- or 15-cis-β-carotene, indicating high specificity for the C9-C10 double bond. The isomerization reaction was inhibited in the presence of silver acetate, pointing to the involvement of an iron-sulfur cluster. We further investigated the expression of AtD27, using Arabidopsis transgenic lines expressing β-glucuronidase (GUS) under the control of AtD27 native promoter. AtD27 is ubiquitously expressed throughout the plant with the highest expression in immature flowers. In lateral roots, AtD27 expression was induced by treatment with auxin and ABA, while the application of SL analogs did not show an effect. Lower ABA levels in atd27 mutant indicated an interference with the ABA pathway. Quantitative real-time RT-PCR showed that transcript levels of AtD27 and other SL biosynthetic genes in roots are induced upon phosphate starvation. Taken together, our study on AtD27 confirms the postulated enzymatic function of this enzyme, shows its strict substrate- and regio-specificity and indicates an important role in response to multiple plant hormones and phosphate deficiency.
dc.description.sponsorshipThis work was supported by base line funding and a Competitive Research Grant, Round 4 (CRG4) from the King Abdullah University of Science and Technology (KAUST).
dc.publisherElsevier BV
dc.relation.urlhttps://www.sciencedirect.com/science/article/pii/S0168945218306022
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Plant Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Plant Science, [, , (2018-09-11)] DOI: 10.1016/j.plantsci.2018.06.024 . © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectArabidopsis
dc.subjectabscisic acid
dc.subjectDWARF27
dc.subjectβ-carotene isomerase
dc.subjectcarotenoids
dc.subjectstrigolactones
dc.titleThe Arabidopsis DWARF27 gene encodes an all-trans-/9-cis-β-carotene isomerase and is induced by auxin, abscisic acid and phosphate deficiency
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentPlant Science
dc.identifier.journalPlant Science
dc.eprint.versionPost-print
kaust.personAbuauf, Haneen Waleed Hamza
kaust.personHaider, Imran
kaust.personJia, Kunpeng
kaust.personAblazov, Abdugaffor
kaust.personMi, Jianing
kaust.personBlilou, Ikram
kaust.personAl-Babili, Salim
refterms.dateFOA2018-09-16T13:02:31Z
dc.date.published-online2018-09-11
dc.date.published-print2018-12


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