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    The Arabidopsis DWARF27 gene encodes an all-trans-/9-cis-β-carotene isomerase and is induced by auxin, abscisic acid and phosphate deficiency

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    Type
    Article
    Authors
    Abuauf, Haneen W. cc
    Haider, Imran cc
    Jia, Kunpeng cc
    Ablazov, Abdugaffor cc
    Mi, Jianing cc
    Blilou, Ikram cc
    Al-Babili, Salim cc
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Bioscience Program
    Center for Desert Agriculture
    Plant Science
    Date
    2018-09-11
    Online Publication Date
    2018-09-11
    Print Publication Date
    2018-12
    Permanent link to this record
    http://hdl.handle.net/10754/628729
    
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    Abstract
    Strigolactones (SLs) are carotenoid-derived plant hormones that influence various aspects of plant growth and development in response to environmental conditions, especially nutrients deficiency. SLs are synthesized via a strict stereo-specific core pathway that leads to the intermediate carlactone, requiring the iron-containing polypeptide DWARF27 (D27) and the carotenoid cleavage dioxygenases 7 (CCD7) and 8 (CCD8). It has been shown that the rice OsD27 is a β-carotene isomerase catalyzing the interconversion of all-trans- into 9-cis-β -carotene. However, data about the enzymatic activity of D27 from other species are missing. Here, we investigated the activity and substrate specificity of the Arabidopsis AtD27 by testing a broad range of carotenoid substrates. Both in vivo and in vitro assays show that AtD27 catalyzes the reverse isomerization of all-trans-/9-cis-β-carotene. AtD27 did not isomerize 13-cis- or 15-cis-β-carotene, indicating high specificity for the C9-C10 double bond. The isomerization reaction was inhibited in the presence of silver acetate, pointing to the involvement of an iron-sulfur cluster. We further investigated the expression of AtD27, using Arabidopsis transgenic lines expressing β-glucuronidase (GUS) under the control of AtD27 native promoter. AtD27 is ubiquitously expressed throughout the plant with the highest expression in immature flowers. In lateral roots, AtD27 expression was induced by treatment with auxin and ABA, while the application of SL analogs did not show an effect. Lower ABA levels in atd27 mutant indicated an interference with the ABA pathway. Quantitative real-time RT-PCR showed that transcript levels of AtD27 and other SL biosynthetic genes in roots are induced upon phosphate starvation. Taken together, our study on AtD27 confirms the postulated enzymatic function of this enzyme, shows its strict substrate- and regio-specificity and indicates an important role in response to multiple plant hormones and phosphate deficiency.
    Citation
    Abuauf H, Haider I, Jia K-P, Ablazov A, Mi J, et al. (2018) The Arabidopsis DWARF27 gene encodes an all-trans-/9-cis-β-carotene isomerase and is induced by auxin, abscisic acid and phosphate deficiency. Plant Science. Available: http://dx.doi.org/10.1016/j.plantsci.2018.06.024.
    Sponsors
    This work was supported by base line funding and a Competitive Research Grant, Round 4 (CRG4) from the King Abdullah University of Science and Technology (KAUST).
    Publisher
    Elsevier BV
    Journal
    Plant Science
    DOI
    10.1016/j.plantsci.2018.06.024
    Additional Links
    https://www.sciencedirect.com/science/article/pii/S0168945218306022
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.plantsci.2018.06.024
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Center for Desert Agriculture

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