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dc.contributor.authorZhang, ShouDong
dc.contributor.authorZhang, Huoming
dc.contributor.authorXia, Yiji
dc.contributor.authorXiong, Liming
dc.date.accessioned2018-09-16T12:40:41Z
dc.date.available2018-09-16T12:40:41Z
dc.date.issued2018-09-12
dc.identifier.citationZhang S, Zhang H, Xia Y, Xiong L (2018) The caseinolytic protease complex component CLPC1 in Arabidopsis maintains proteome and RNA homeostasis in chloroplasts. BMC Plant Biology 18. Available: http://dx.doi.org/10.1186/s12870-018-1396-0.
dc.identifier.issn1471-2229
dc.identifier.doi10.1186/s12870-018-1396-0
dc.identifier.urihttp://hdl.handle.net/10754/628727
dc.description.abstractHomeostasis of the proteome is critical to the development of chloroplasts and also affects the expression of certain nuclear genes. CLPC1 facilitates the translocation of chloroplast pre-proteins and mediates protein degradation.We found that proteins involved in photosynthesis are dramatically decreased in their abundance in the clpc1 mutant, whereas many proteins involved in chloroplast transcription and translation were increased in the mutant. Expression of the full-length CLPC1 protein, but not of the N-terminus-deleted CLPC1 (ΔN), in the clpc1 mutant background restored the normal levels of most of these proteins. Interestingly, the ΔN complementation line could also restore some proteins affected by the mutation to normal levels. We also found that that the clpc1 mutation profoundly affects transcript levels of chloroplast genes. Sense transcripts of many chloroplast genes are up-regulated in the clpc1 mutant. The level of SVR7, a PPR protein, was affected by the clpc1 mutation. We showed that SVR7 might be a target of CLPC1 as CLPC1-SVR7 interaction was detected through co-immunoprecipitation.Our study indicates that in addition to its role in maintaining proteome homeostasis, CLPC1 and likely the CLP proteasome complex also play a role in transcriptome homeostasis through its functions in maintaining proteome homeostasis.
dc.description.sponsorshipAcknowledgements: The authors thank Prof. Hsou-min Li (Institute of Molecular Biology, Taiwan) for clpc1, ΔN and CP lines and Prof. Paul Jarvis (Department of Plant Science, Oxford University, U.K) for 1.4.3 and 1.4.4 lines used in the work. We also thank the KAUST Bioscience Core Facility for quantitative proteomics, we also thank the PRIDE Team to help us deposit the raw data to proteomeXchange. Funding: This work was supported by King Abdullah University of Science and Technology Baseline Grant BAS/1/1007-01-01 (to L.X), Hong Kong RGC GRF fund (GRF 12100717 to SZ), Hong Kong Baptist University FRG2/16–17/026 (to SZ), and HKBU Strategic Development Fund SDF 15–1012-P04 (to YX) as well as Hong Kong Research Grants Council Area of Excellent scheme, Grant/Award Number: AoE/M-403/16.
dc.publisherSpringer Nature
dc.relation.urlhttps://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-018-1396-0
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectChloroplast
dc.subjectProteome
dc.subjectHomeostasis
dc.subjectTranscriptome
dc.subjectClpc1
dc.subjectSvr7
dc.titleThe caseinolytic protease complex component CLPC1 in Arabidopsis maintains proteome and RNA homeostasis in chloroplasts
dc.typeArticle
dc.contributor.departmentAcademic Affairs
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentCore Labs
dc.contributor.departmentOffice of the VP
dc.contributor.departmentPlant Science
dc.contributor.departmentPlant Stress Genomics Research Lab
dc.contributor.departmentProteomics, protein expression & cytomet
dc.identifier.journalBMC Plant Biology
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionPartner State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Shatin, Hong Kong SAR, China.
dc.contributor.institutionDepartment of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China.
dc.contributor.institutionPartner State Key Laboratory of Agrobiotechnology, Chinese University of Hong Kong, Shatin, Hong Kong, SAR, China.
dc.contributor.institutionDepartment of Horticultural Sciences, Texas A&M University, College Station, TX 77843, USA.
dc.contributor.institutionTexas A&M AgriLife Research Center, Dallas, TX 75252, USA.
kaust.personZhang, ShouDong
kaust.personZhang, Huoming
kaust.personXiong, Liming
kaust.grant.numberBAS/1/1007-01-01
dc.relation.issupplementedbyDOI:10.6084/m9.figshare.c.4230722
dc.relation.issupplementedbyDOI:10.6084/m9.figshare.7081469.v1
refterms.dateFOA2018-09-16T13:10:07Z
display.relations<b> Is Supplemented By:</b> <br/> <ul> <li><i>[Dataset]</i> <br/> . DOI: <a href="https://doi.org/10.6084/m9.figshare.c.4230722">10.6084/m9.figshare.c.4230722</a> HANDLE: <a href="http://hdl.handle.net/10754/664189">10754/664189</a></li></ul><b> Is Supplemented By:</b> <br/> <ul> <li><i>[Dataset]</i> <br/> . DOI: <a href="https://doi.org/10.6084/m9.figshare.7081469.v1">10.6084/m9.figshare.7081469.v1</a> HANDLE: <a href="http://hdl.handle.net/10754/664190">10754/664190</a></li></ul>
dc.date.published-online2018-09-12
dc.date.published-print2018-12


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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Except where otherwise noted, this item's license is described as This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.