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dc.contributor.authorYang, Ping
dc.contributor.authorPraz, Coraline
dc.contributor.authorLi, Beibei
dc.contributor.authorSingla, Jyoti
dc.contributor.authorRobert, Christelle A. M.
dc.contributor.authorKessel, Bettina
dc.contributor.authorScheuermann, Daniela
dc.contributor.authorLüthi, Linda
dc.contributor.authorOuzunova, Milena
dc.contributor.authorErb, Matthias
dc.contributor.authorKrattinger, Simon G.
dc.contributor.authorKeller, Beat
dc.date.accessioned2018-09-09T12:27:36Z
dc.date.available2018-09-09T12:27:36Z
dc.date.issued2018-09-04
dc.identifier.citationYang P, Praz C, Li B, Singla J, Robert CAM, et al. (2018) Fungal resistance mediated by maize wall-associated kinase ZmWAK-RLK1 correlates with reduced benzoxazinoid content. New Phytologist. Available: http://dx.doi.org/10.1111/nph.15419.
dc.identifier.issn0028-646X
dc.identifier.doi10.1111/nph.15419
dc.identifier.urihttp://hdl.handle.net/10754/628523
dc.description.abstractWall-associated kinases (WAKs) have recently been identified as major components of fungal and bacterial disease resistance in several cereal crop species. However, the molecular mechanisms of WAK-mediated resistance remain largely unknown. \nHere, we investigated the function of the maize gene ZmWAK-RLK1 (Htn1) that confers quantitative resistance to northern corn leaf blight (NCLB) caused by the hemibiotrophic fungal pathogen Exserohilum turcicum. \nZmWAK-RLK1 was found to localize to the plasma membrane and its presence resulted in a modification of the infection process by reducing pathogen penetration into host tissues. A large-scale transcriptome analysis of near-isogenic lines (NILs) differing for ZmWAK-RLK1 revealed that several differentially expressed genes are involved in the biosynthesis of the secondary metabolites benzoxazinoids (BXs). The contents of several BXs including DIM2BOA-Glc were significantly lower when ZmWAK-RLK1 is present. DIM2BOA-Glc concentration was significantly elevated in ZmWAK-RLK1 mutants with compromised NCLB resistance. Maize mutants that were affected in overall BXs biosynthesis or content of DIM2BOA-Glc showed increased NCLB resistance. \nWe conclude that Htn1-mediated NCLB resistance is associated with a reduction of BX secondary metabolites. These findings suggest a link between WAK-mediated quantitative disease resistance and changes in biochemical fluxes starting with indole-3-glycerol phosphate.
dc.description.sponsorshipThe authors would like to thank Dr Severine Hurni (UZH) for helpful discussions, Professor Jiaqiang Sun (Institute of Crop Sciences, CAAS) for assistance in performance of Western blot, Professor Jianfeng Wen for assistance in performance of experiments with maize protoplasts, Professor Georg Jander (Cornell University) for kindly providing bx mutants, and Mr Alessandro Artemisio, Mr Gerhard Herren, Mr Karl Huwiler, Mr Thibault Vassor and Ms Matisse Petit-Prost for technical support. This work was supported by Swiss National Science Foundation Grant 310030_163260 to B Keller. SGK is supported by an Ambizione grant of the Swiss National Science Foundation. PY is supported by Agricultural Science and Technology Innovation Program of CAAS and Fundamental Research Funds for Central Non-Profit of Institute of Crop Sciences of CAAS, China.
dc.language.isoen
dc.publisherWiley
dc.relation.urlhttps://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.15419
dc.rightsArchived with thanks to New Phytologist
dc.subjectbenzoxazinoids (BXs)
dc.subjectHtn1
dc.subjectmaize disease resistance
dc.subjectreceptor-like kinase
dc.subjectwall-associated kinase (WAK)
dc.titleFungal resistance mediated by maize wall-associated kinase ZmWAK-RLK1 correlates with reduced benzoxazinoid content
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentPlant Science
dc.identifier.journalNew Phytologist
dc.eprint.versionPost-print
dc.contributor.institutionInstitute of Crop Sciences; Chinese Academy of Agricultural Sciences; 100081 Beijing China
dc.contributor.institutionDepartment of Plant and Microbial Biology; University of Zurich; Zollikerstrasse 107 CH-8008 Zurich Switzerland
dc.contributor.institutionInstitute of Plant Sciences; University of Bern; CH-3013 Bern Switzerland
dc.contributor.institutionKWS SAAT SE; DE-37574 Einbeck Germany
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personKrattinger, Simon G.
refterms.dateFOA2018-09-09T12:27:36Z
dc.source.journaltitleNew Phytologist
dc.date.published-online2018-09-04
dc.date.published-print2019-01


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