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dc.contributor.authorHoratz, Kilian
dc.contributor.authorGiampà, Marco
dc.contributor.authorKarpov, Yevhen
dc.contributor.authorSahre, Karin
dc.contributor.authorBednarz, Hanna
dc.contributor.authorKiriy, Anton
dc.contributor.authorVoit, Brigitte
dc.contributor.authorNiehaus, Karsten
dc.contributor.authorHadjichristidis, Nikos
dc.contributor.authorMichels, Dominik L.
dc.contributor.authorLissel, Franziska
dc.date.accessioned2018-09-03T13:28:01Z
dc.date.available2018-09-03T13:28:01Z
dc.date.issued2018-08-08
dc.identifier.citationHoratz K, Giampà M, Karpov Y, Sahre K, Bednarz H, et al. (2018) Conjugated Polymers as a New Class of Dual-Mode Matrices for MALDI Mass Spectrometry and Imaging. Journal of the American Chemical Society. Available: http://dx.doi.org/10.1021/jacs.8b06637.
dc.identifier.issn0002-7863
dc.identifier.issn1520-5126
dc.identifier.doi10.1021/jacs.8b06637
dc.identifier.urihttp://hdl.handle.net/10754/628496
dc.description.abstractMatrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) and MALDI MS imaging are ubiquitous analytical methods in medical, pharmaceutical, biological, and environmental research. Currently, there is a strong interest in the investigation of low molecular weight compounds (LMWCs), especially to trace and understand metabolic pathways, requiring the development of new matrix systems that have favorable optical properties and a high ionization efficiency and that are MALDI silent in the LMWC area. In this paper, five conjugated polymers, poly{[ N, N'-bis(2-octyldodecyl)-naphtalene-1,4,5,8-bis(dicarboximide)-2,6-diyl]- alt-5,5'(2,2'-bithiophene)} (PNDI(T2)), poly(3-dodecylthiophene-2,5-diyl) (P3DDT), poly{[2,3-bis(3-octyloxyphenyl)quinoxaline-5,8-diyl]- alt-(thiophene-2,5-diyl)} (PTQ1), poly{[ N, N'-bis(2-octyldodecyl)-isoindigo-5,5'-diyl] -alt-5,5'(2,2'-bithiophene)} (PII(T2)), and poly(9,9-di- n-octylfluorenyl-2,7-diyl) (P9OFl) are investigated as matrices. The polymers have a strong optical absorption, are solution processable, and can be coated into thin films, allowing a vast reduction in the amount of matrix used. All investigated polymers function as matrices in both positive and negative mode MALDI, classifying them as rare dual-mode matrices, and show a very good analyte ionization ability in both modes. PNDI(T2), P3DDT, PTQ1, and PII(T2) are MALDI silent in the full measurement range (> m/ z = 150k), except at high laser intensities. In MALDI MS experiments of single analytes and a complex biological sample, the performance of the polymers was found to be as good as two commonly used matrices (2,5-DHB for positive and 9AA for negative mode measurements). The detection limit of two standard analytes was determined as being below 164 pmol for reserpine and below 245 pmol for cholic acid. Additionally P3DDT was used successfully in first MALDI MS imaging experiments allowing the visualization of the tissue morphology of rat brain sections.
dc.description.sponsorshipThe work was supported by KAUST Center Partnership Fund OSR-2017-CPF-3322-01. F.L. thanks the VCI for a Liebig Fellowship. The authors gratefully acknowledge the support of Wolfgang Heidrich in establishing the collaboration infrastructure between KAUST’s VCC and the IPF. Anastasia Khrenova is thanked for helping with the graphics.
dc.publisherAmerican Chemical Society (ACS)
dc.relation.urlhttps://pubs.acs.org/doi/10.1021/jacs.8b06637
dc.rightsThis is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
dc.rights.urihttp://pubs.acs.org/page/policy/authorchoice_termsofuse.html
dc.titleConjugated Polymers as a New Class of Dual-Mode Matrices for MALDI Mass Spectrometry and Imaging
dc.typeArticle
dc.contributor.departmentChemical Science Program
dc.contributor.departmentComputer Science Program
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentKAUST Catalysis Center (KCC)
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.contributor.departmentPolymer Synthesis Laboratory
dc.contributor.departmentVisual Computing Center (VCC)
dc.identifier.journalJournal of the American Chemical Society
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionOrganic Chemistry of Polymers, Technische Universität Dresden, Mommsenstrasse 4, 01062 Dresden, Germany
dc.contributor.institutionInstitute of Macromolecular Chemistry, Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Straße 6, 01069 Dresden, Germany
dc.contributor.institutionCenter for Biotechnology and Department for Proteome and Metabolome Research, Faculty of Biology, Bielefeld University, Universitätsstraße 25, 33615 Bielefeld, Germany
kaust.personHadjichristidis, Nikos
kaust.personMichels, Dominik L.
kaust.grant.numberOSR-2017-CPF-3322-01
refterms.dateFOA2018-09-04T07:00:07Z
dc.date.published-online2018-08-08
dc.date.published-print2018-09-12


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