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dc.contributor.authorLi, Yong
dc.contributor.authorLiew, Yi Jin
dc.contributor.authorCui, Guoxin
dc.contributor.authorCziesielski, Maha Joana
dc.contributor.authorZahran, Noura Ibrahim Omar
dc.contributor.authorMichell, Craig
dc.contributor.authorVoolstra, Christian R.
dc.contributor.authorAranda, Manuel
dc.date.accessioned2018-09-03T13:26:57Z
dc.date.available2018-09-03T13:26:57Z
dc.date.issued2018-08-15
dc.identifier.citationLi Y, Liew YJ, Cui G, Cziesielski MJ, Zahran N, et al. (2018) DNA methylation regulates transcriptional homeostasis of algal endosymbiosis in the coral model Aiptasia. Science Advances 4: eaat2142. Available: http://dx.doi.org/10.1126/sciadv.aat2142.
dc.identifier.issn2375-2548
dc.identifier.doi10.1126/sciadv.aat2142
dc.identifier.urihttp://hdl.handle.net/10754/628481
dc.description.abstractThe symbiotic relationship between cnidarians and dinoflagellates is the cornerstone of coral reef ecosystems. Although research has focused on the molecular mechanisms underlying this symbiosis, the role of epigenetic mechanisms, that is, the study of heritable changes that do not involve changes in the DNA sequence, is unknown. To assess the role of DNA methylation in the cnidarian-dinoflagellate symbiosis, we analyzed genome-wide CpG methylation, histone associations, and transcriptomic states of symbiotic and aposymbiotic anemones in the model system Aiptasia. We found that methylated genes are marked by histone 3 lysine 36 trimethylation (H3K36me3) and show significant reduction of spurious transcription and transcriptional noise, revealing a role of DNA methylation in the maintenance of transcriptional homeostasis. Changes in DNA methylation and expression show enrichment for symbiosis-related processes, such as immunity, apoptosis, phagocytosis recognition, and phagosome formation, and reveal intricate interactions between the underlying pathways. Our results demonstrate that DNA methylation provides an epigenetic mechanism of transcriptional homeostasis that responds to symbiosis.
dc.description.sponsorshipAcknowledgments: We thank J. Pringle for the provision of the initial Aiptasia CC7 and Symbiodinium SSB01 cultures. We thank S. Baumgarten for his help with establishing the Aiptasia cultures and critical reading of the manuscript. We also thank the four anonymous reviewers for their effort and valuable suggestions, which greatly improved this study. Funding: Research reported in this publication was supported by baseline funding from the King Abdullah University of Science and Technology to M.A.
dc.publisherAmerican Association for the Advancement of Science (AAAS)
dc.relation.urlhttp://advances.sciencemag.org/content/4/8/eaat2142
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.titleDNA methylation regulates transcriptional homeostasis of algal endosymbiosis in the coral model Aiptasia
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentMarine Science Program
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.identifier.journalScience Advances
dc.eprint.versionPublisher's Version/PDF
kaust.personLi, Yong
kaust.personLiew, Yi Jin
kaust.personCui, Guoxin
kaust.personCziesielski Olschowsky, Maha Joana
kaust.personZahran, Noura Ibrahim Omar
kaust.personMichell, Craig
kaust.personVoolstra, Christian R.
kaust.personAranda, Manuel
dc.relation.issupplementedbybioproject:PRJNA415358
dc.relation.issupplementedbygithub:YongLiedu/Aiptasia_DNA_Meth
refterms.dateFOA2018-09-04T11:49:59Z
display.relations<b>Is Supplemented By:</b><br/> <ul><li><i>[Bioproject]</i> <br/> Title: Aiptasia whole-genome bisulfite sequencing (DNA methylation) on cnidarian-dinoflagellate symbiosis. Publication Date: 2017-10-23. bioproject: <a href="https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA415358" >PRJNA415358</a> Handle: <a href="http://hdl.handle.net/10754/666486" >10754/666486</a></a></li><li><i>[Software]</i> <br/> Title: YongLiedu/Aiptasia_DNA_Meth: Here are some codes for DNA methylation analysis.. Publication Date: 2018-05-06. github: <a href="https://github.com/YongLiedu/Aiptasia_DNA_Meth" >YongLiedu/Aiptasia_DNA_Meth</a> Handle: <a href="http://hdl.handle.net/10754/666972" >10754/666972</a></a></li></ul>
dc.date.published-online2018-08-15
dc.date.published-print2018-08


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This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.