CRISPR base editors: genome editing without double-stranded breaks
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Desert Agriculture Initiative
Lab Consumables & Gases
Laboratory for Genome Engineering
Online Publication Date2018-06-11
Print Publication Date2018-06-15
Permanent link to this recordhttp://hdl.handle.net/10754/628449
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AbstractThe CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 adaptive immunity system has been harnessed for genome editing applications across eukaryotic species, but major drawbacks, such as the inefficiency of precise base editing and offtarget activities, remain. A catalytically inactive Cas9 variant (dead Cas9, dCas9) has been fused to diverse functional domains for targeting genetic and epigenetic modifications, including base editing, to specific DNA sequences. As base editing does not require the generation of double-strand breaks, dCas9 and Cas9 nickase have been used to target deaminase domains to edit specific loci. Adenine and cytidine deaminases convert their respective nucleotides into other DNA bases, thereby offering many possibilities for DNA editing. Such base-editing enzymes hold great promise for applications in basic biology, trait development in crops, and treatment of genetic diseases. Here, we discuss recent advances in precise gene editing using different platforms as well as their potential applications in basic biology and biotechnology.
CitationEid A, Alshareef S, Mahfouz MM (2018) CRISPR base editors: genome editing without double-stranded breaks. Biochemical Journal 475: 1955–1964. Available: http://dx.doi.org/10.1042/bcj20170793.
SponsorsResearch in the M.M.M.'s laboratory for genome engineering is supported by the King Abdullah University of Science and Technology. We thank members of the genome-engineering laboratory at KAUST for discussions.
PublisherPortland Press Ltd.
Except where otherwise noted, this item's license is described as This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY-NC-ND).