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dc.contributor.authorCastro, David
dc.contributor.authorConchouso Gonzalez, David
dc.contributor.authorKodzius, Rimantas
dc.contributor.authorCarreno, Armando Arpys Arevalo
dc.contributor.authorFoulds, Ian G.
dc.date.accessioned2018-09-03T13:23:00Z
dc.date.available2018-09-03T13:23:00Z
dc.date.issued2018-06-04
dc.identifier.citationCastro D, Conchouso D, Kodzius R, Arevalo A, Foulds I (2018) High-Throughput Incubation and Quantification of Agglutination Assays in a Microfluidic System. Genes 9: 281. Available: http://dx.doi.org/10.3390/genes9060281.
dc.identifier.issn2073-4425
dc.identifier.doi10.3390/genes9060281
dc.identifier.urihttp://hdl.handle.net/10754/628428
dc.description.abstractIn this paper, we present a two-phase microfluidic system capable of incubating and quantifying microbead-based agglutination assays. The microfluidic system is based on a simple fabrication solution, which requires only laboratory tubing filled with carrier oil, driven by negative pressure using a syringe pump. We provide a user-friendly interface, in which a pipette is used to insert single droplets of a 1.25-µL volume into a system that is continuously running and therefore works entirely on demand without the need for stopping, resetting or washing the system. These assays are incubated by highly efficient passive mixing with a sample-to-answer time of 2.5 min, a 5–10-fold improvement over traditional agglutination assays. We study system parameters such as channel length, incubation time and flow speed to select optimal assay conditions, using the streptavidin-biotin interaction as a model analyte quantified using optical image processing. We then investigate the effect of changing the concentration of both analyte and microbead concentrations, with a minimum detection limit of 100 ng/mL. The system can be both low-and high-throughput, depending on the rate at which assays are inserted. In our experiments, we were able to easily produce throughputs of 360 assays per hour by simple manual pipetting, which could be increased even further by automation and parallelization. Agglutination assays are a versatile tool, capable of detecting an ever-growing catalog of infectious diseases, proteins and metabolites. A system such as this one is a step towards being able to produce high-throughput microfluidic diagnostic solutions with widespread adoption. The development of analytical techniques in the microfluidic format, such as the one presented in this work, is an important step in being able to continuously monitor the performance and microfluidic outputs of organ-on-chip devices.
dc.description.sponsorshipThis research did not receive any specific grant from funding agencies in the public, commercial or not-for-profit sectors.
dc.publisherMDPI AG
dc.relation.urlhttp://www.mdpi.com/2073-4425/9/6/281/htm
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAgglutination assay
dc.subjectHigh-throughput
dc.subjectLab-on-chip
dc.subjectMicrofluidics
dc.titleHigh-Throughput Incubation and Quantification of Agglutination Assays in a Microfluidic System
dc.typeArticle
dc.contributor.departmentCompetitive Research Funds
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentElectrical Engineering Program
dc.contributor.departmentMechanical Engineering Program
dc.contributor.departmentOCRF- Special Academic Partnership
dc.contributor.departmentOffice of the VP
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalGenes
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionFaculty of Medicine, Ludwig Maximilian University of Munich (LMU), Munich, 80539, , Germany
dc.contributor.institutionMathematics and Natural Sciences Department, The American University of Iraq, Sulaimani, Sulaymaniyah, 46001, , Iraq
dc.contributor.institutionOkanagan Campus, School of Engineering, Faculty of Applied Science, University of British Columbia, 3333 University Way, Kelowna, BC, V1V 1V7, , Canada
kaust.personCastro, David
kaust.personConchouso Gonzalez, David
kaust.personKodzius, Rimantas
kaust.personCarreno, Armando Arpys Arevalo
kaust.personFoulds, Ian G.
refterms.dateFOA2018-09-09T13:34:44Z


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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
Except where otherwise noted, this item's license is described as This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).