Direct metabolite detection with an n-type accumulation mode organic electrochemical transistor
AuthorsPappa, Anna Maria
Giovannitti , Alexander
Maria, Iuliana Petruta
Owens, Róisín M.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Chemical Science Program
KAUST Solar Center (KSC)
Physical Science and Engineering (PSE) Division
KAUST Grant NumberOSR-2016-CRG5-3003
Online Publication Date2018-06-22
Print Publication Date2018-06
Permanent link to this recordhttp://hdl.handle.net/10754/628423
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AbstractThe inherent specificity and electrochemical reversibility of enzymes poise them as the biorecognition element of choice for a wide range of metabolites. To use enzymes efficiently in biosensors, the redox centers of the protein should have good electrical communication with the transducing electrode, which requires either the use of mediators or tedious biofunctionalization approaches. We report an all-polymer micrometer-scale transistor platform for the detection of lactate, a significant metabolite in cellular metabolic pathways associated with critical health care conditions. The device embodies a new concept in metabolite sensing where we take advantage of the ion-to-electron transducing qualities of an electron-transporting (n-type) organic semiconductor and the inherent amplification properties of an ion-to-electron converting device, the organic electrochemical transistor. The n-type polymer incorporates hydrophilic side chains to enhance ion transport/ injection, as well as to facilitate enzyme conjugation. The material is capable of accepting electrons of the enzymatic reaction and acts as a series of redox centers capable of switching between the neutral and reduced state. The result is a fast, selective, and sensitive metabolite sensor. The advantage of this device compared to traditional amperometric sensors is the amplification of the input signal endowed by the electrochemical transistor circuit and the design simplicity obviating the need for a reference electrode. The combination of redox enzymes and electron-transporting polymers will open up an avenue not only for the field of biosensors but also for the development of enzyme-based electrocatalytic energy generation/storage devices.
CitationPappa AM, Ohayon D, Giovannitti A, Maria IP, Savva A, et al. (2018) Direct metabolite detection with an n-type accumulation mode organic electrochemical transistor. Science Advances 4: eaat0911. Available: http://dx.doi.org/10.1126/sciadv.aat0911.
SponsorsA.M.P. and R.M.O. acknowledge the support by the Marie Curie Innovative Training Networks project OrgBio no. 607896. D.O., I.M., and S.I. acknowledge financial support from the King Abdullah University of Science and Technology Office of Sponsored Research (OSR) under award no. OSR-2016-CRG5-3003. A.G. and I.M. acknowledge funding from Engineering and Physical Sciences Research Council Project EP/G037515/1 and EP/N509486/1.
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