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dc.contributor.authorKavaldzhiev, Mincho
dc.contributor.authorPerez, Jose E.
dc.contributor.authorSougrat, Rachid
dc.contributor.authorBergam, Ptissam
dc.contributor.authorRavasi, Timothy
dc.contributor.authorKosel, Jürgen
dc.date.accessioned2018-09-03T13:21:01Z
dc.date.available2018-09-03T13:21:01Z
dc.date.issued2018-07-02
dc.identifier.citationKavaldzhiev MN, Perez JE, Sougrat R, Bergam P, Ravasi T, et al. (2018) Inductively actuated micro needles for on-demand intracellular delivery. Scientific Reports 8. Available: http://dx.doi.org/10.1038/s41598-018-28194-3.
dc.identifier.issn2045-2322
dc.identifier.doi10.1038/s41598-018-28194-3
dc.identifier.urihttp://hdl.handle.net/10754/628400
dc.description.abstractMethods that provide controlled influx of molecules into cells are of critical importance for uncovering cellular mechanisms, drug development and synthetic biology. However, reliable intracellular delivery without adversely affecting the cells is a major challenge. We developed a platform for on-demand intracellular delivery applications, with which cell membrane penetration is achieved by inductive heating of micro needles. The micro needles of around 1 μm in diameter and 5 μm in length are made of gold using a silicon-based micro fabrication process that provides flexibility with respect to the needles' dimensions, pitch, shell thickness and the covered area. Experiments with HCT 116 colon cancer cells showed a high biocompatibility of the gold needle platform. Transmission electron microscopy of the cell-needle interface revealed folding of the cell membrane around the needle without penetration, preventing any delivery, which was confirmed using the EthD-1 fluorescent dye. The application of an alternating magnetic field, however, resulted in the delivery of EthD-1 by localized heating of the micro needles. Fluorescence quantification showed that intracellular delivery, with as high as 75% efficiency, is achieved for specific treatment times between 1 and 5 minutes. Overexposure of the cells to the heated micro needles, i.e. longer magnetic field application, leads to an increase in cell death, which can be exploited for cleaning the platform. This method allows to perform intracellular deliver by remotely activating the micro needles via a magnetic field, and it is controlled by the application time, making it a versatile and easy to use method. The wireless actuation could also be an attractive feature for in-vivo delivery and implantable devices.
dc.description.sponsorshipThis work was funded by King Abdullah University of Science and Technology. This work would not have been possible without the contribution of Ahad Syed and Zhihong Wang from the nanofab core lab at KAUST.
dc.publisherSpringer Nature
dc.relation.urlhttps://www.nature.com/articles/s41598-018-28194-3
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleInductively actuated micro needles for on-demand intracellular delivery
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentChemical and Biological Engineering Program
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentElectrical Engineering Program
dc.contributor.departmentElectron Microscopy
dc.contributor.departmentImaging and Characterization Core Lab
dc.identifier.journalScientific Reports
dc.eprint.versionPublisher's Version/PDF
kaust.personKavaldzhiev, Mincho
kaust.personPerez, Jose E.
kaust.personSougrat, Rachid
kaust.personBergam, Ptissam
kaust.personRavasi, Timothy
kaust.personKosel, Jürgen
refterms.dateFOA2018-09-10T14:01:18Z
dc.date.published-online2018-07-02
dc.date.published-print2018-12


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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Except where otherwise noted, this item's license is described as This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.