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dc.contributor.authorCali, Corrado
dc.contributor.authorWawrzyniak, Marta
dc.contributor.authorBecker, Carlos
dc.contributor.authorMaco, Bohumil
dc.contributor.authorCantoni, Marco
dc.contributor.authorJorstad, Anne
dc.contributor.authorNigro, Biagio
dc.contributor.authorGrillo, Federico
dc.contributor.authorDe Paola, Vincenzo
dc.contributor.authorFua, Pascal
dc.contributor.authorKnott, Graham William
dc.date.accessioned2018-09-03T13:19:41Z
dc.date.available2018-09-03T13:19:41Z
dc.date.issued2018-07-02
dc.identifier.citationCalì C, Wawrzyniak M, Becker C, Maco B, Cantoni M, et al. (2018) The effects of aging on neuropil structure in mouse somatosensory cortex—A 3D electron microscopy analysis of layer 1. PLOS ONE 13: e0198131. Available: http://dx.doi.org/10.1371/journal.pone.0198131.
dc.identifier.issn1932-6203
dc.identifier.doi10.1371/journal.pone.0198131
dc.identifier.urihttp://hdl.handle.net/10754/628380
dc.description.abstractThis study has used dense reconstructions from serial EM images to compare the neuropil ultrastructure and connectivity of aged and adult mice. The analysis used models of axons, dendrites, and their synaptic connections, reconstructed from volumes of neuropil imaged in layer 1 of the somatosensory cortex. This shows the changes to neuropil structure that accompany a general loss of synapses in a well-defined brain region. The loss of excitatory synapses was balanced by an increase in their size such that the total amount of synaptic surface, per unit length of axon, and per unit volume of neuropil, stayed the same. There was also a greater reduction of inhibitory synapses than excitatory, particularly those found on dendritic spines, resulting in an increase in the excitatory/inhibitory balance. The close correlations, that exist in young and adult neurons, between spine volume, bouton volume, synaptic size, and docked vesicle numbers are all preserved during aging. These comparisons display features that indicate a reduced plasticity of cortical circuits, with fewer, more transient, connections, but nevertheless an enhancement of the remaining connectivity that compensates for a generalized synapse loss.
dc.description.sponsorshipThe study was supported by a Novartis Biomedical Foundation grant, and a Swiss National Science Foundation grant to Graham Knott. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
dc.publisherPublic Library of Science (PLoS)
dc.relation.urlhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0198131
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleThe effects of aging on neuropil structure in mouse somatosensory cortex—A 3D electron microscopy analysis of layer 1
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalPLOS ONE
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionBioEM Facility, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, , Switzerland
dc.contributor.institutionBioEPIX, XLI M, Faculty of Science and Technology, University of Limoges, Limoges, , France
dc.contributor.institutionComputer Vision Laboratory, Ecole Polytechnique Fédérale de Lausanne, Lausanne, , Switzerland
dc.contributor.institutionCentre for Electron Microscopy, Ecole Polytechnique Fédérale de Lausanne, Lausanne, , Switzerland
dc.contributor.institutionMRC Centre for Developmental Neurobiology, King’s College London, New Hunt’s House Guy’s Campus, London, , United Kingdom
dc.contributor.institutionMRC Clinical Science Centre, Faculty of Medicine, Imperial College London, London, , , United Kingdom
kaust.personCali, Corrado
dc.relation.issupplementedbyDOI:10.5061/dryad.bh78sn5
refterms.dateFOA2018-09-11T13:33:09Z
display.relations<b>Is Supplemented By:</b><br/> <ul><li><i>[Dataset]</i> <br/> Cali, C., Wawrzyniak, M., Becker, C., Maco, B., Cantoni, M., Jorstad, A., Nigro, B., Grillo, F., De Paola, V., Fua, P., &amp; Knott, G. W. (2019). Data from: The effects of aging on neuropil structure in mouse somatosensory cortex—A 3D electron microscopy analysis of layer 1 (Version 1) [Data set]. Dryad. https://doi.org/10.5061/DRYAD.BH78SN5. DOI: <a href="https://doi.org/10.5061/dryad.bh78sn5" >10.5061/dryad.bh78sn5</a> Handle: <a href="http://hdl.handle.net/10754/662363" >10754/662363</a></a></li></ul>


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This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.