Aggregation ability of three phylogenetically distant anammox bacterial species
Shaw, Dario Rangel
Haroon, Mohamed Fauzi
Emwas, Abdul-Hamid M.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Environmental Science and Engineering Program
Water Desalination and Reuse Research Center (WDRC)
KAUST Grant NumberCRG_R2_13_SAIK_KAUST_1
Online Publication Date2018-06-12
Print Publication Date2018-10
Permanent link to this recordhttp://hdl.handle.net/10754/628290
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AbstractAnaerobic ammonium-oxidizing (anammox) bacteria are well known for their aggregation ability. However, very little is known about cell surface physicochemical properties of anammox bacteria and thus their aggregation abilities have not been quantitatively evaluated yet. Here, we investigated the aggregation abilities of three different anammox bacterial species: “Candidatus Brocadia sinica”, “Ca. Jettenia caeni” and “Ca. Brocadia sapporoensis”. Planktonic free-living enrichment cultures of these three anammox species were harvested from the membrane bioreactors (MBRs). The physicochemical properties (e.g., contact angle, zeta potential, and surface thermodynamics) were analyzed for these anammox bacterial species and used in the extended DLVO theory to understand the force-distance relationship. In addition, their extracellular polymeric substances (EPSs) were characterized by X-ray photoelectron spectroscopy and nuclear magnetic resonance. The results revealed that the “Ca. B. sinica” cells have the most hydrophobic surface and less hydrophilic functional groups in EPS than other anammox strains, suggesting better aggregation capability. Furthermore, aggregate formation and anammox bacterial populations were monitored when planktonic free-living cells were cultured in up-flow column reactors under the same conditions. Rapid development of microbial aggregates was observed with the anammox bacterial population shifts to a dominance of “Ca. B. sinica” in all three reactors. The dominance of “Ca. B. sinica” could be explained by its better aggregation ability and the superior growth kinetic properties (higher growth rate and affinity to nitrite). The superior aggregation ability of “Ca. B. sinica” indicates significant advantages (efficient and rapid start-up of anammox reactors due to better biomass retention as granules and consequently stable performance) in wastewater treatment application.
CitationAli M, Shaw DR, Zhang L, Haroon MF, Narita Y, et al. (2018) Aggregation ability of three phylogenetically distant anammox bacterial species. Water Research 143: 10–18. Available: http://dx.doi.org/10.1016/j.watres.2018.06.007.
SponsorsThis research financially supported by Nagase Science and Technology Foundation and Institute for Fermentation, Osaka (IFO), which were granted to Satoshi Okabe, and by Competitive Research Grant (CRG_R2_13_SAIK_KAUST_1) from King Abdullah University of Science and Technology (KAUST). Lei Zhang was supported partly by the Monbukagakusho Honors Scholarship for Privately Financed International Students offered by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. When Muhammad Ali was a Post-Doctoral Fellow at Hokkaido University, Sapporo Japan, he was supported by Graduate School of Engineering, Hokkaido University, Japan.