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dc.contributor.authorHe, Song
dc.contributor.authorHarrison, Hugo B.
dc.contributor.authorBerumen, Michael L.
dc.date.accessioned2018-06-03T10:43:15Z
dc.date.available2018-06-03T10:43:15Z
dc.date.issued2018-06-01
dc.identifier.citationHe S, Harrison HB, Berumen ML (2018) Species delineation and hybrid identification using diagnostic nuclear markers for Plectropomus leopardus and Plectropomus maculatus. Fisheries Research 206: 287–291. Available: http://dx.doi.org/10.1016/j.fishres.2018.05.022.
dc.identifier.issn0165-7836
dc.identifier.doi10.1016/j.fishres.2018.05.022
dc.identifier.urihttp://hdl.handle.net/10754/628018
dc.description.abstractDiagnostic molecular markers are an essential tool in the study of species’ ecology and evolution, particularly in closely related and sympatric species. Furthermore, the increasing awareness of wild-hybrids has led to a renewed interest in rapid diagnostic assays. Here, we test the ability of two mitochondrial (Cytb and COI) and two nuclear markers (ETS2 and TMO-4c4) to confidently discriminate purebred P. leopardus and P. maculatus and their first-generation hybrids. A sample of 48 purebred individuals and 91 interspecific hybrids were used in this study and their delineation confirmed using a set of microsatellite markers. Our results indicate mitochondrial markers could not distinguish even between species but both nuclear markers confidently identified species and first-generation hybrids. However, later-generation hybrids could not always be confidently identified due to on-going introgression between species. Our findings provide a robust tool to distinguish purebred individuals and interspecific hybrids in a pair of species with an unexpectedly high incidence of hybridization. The quick species discrimination abilities provided by these diagnostic markers are important for stock assessment and recruitment studies of these important fishery species.
dc.description.sponsorshipWe thank King Abdullah and University of Science and Technology (KAUST) Bioscience Core Laboratory for laboratory support. We acknowledge Jean-François Flot and Tane Sinclair-Taylor for helpful discussions and assistance with figures. Financial support was provided by KAUST baseline research funds to M. L. B. We also thank anonymous reviewers for their constructive comments.
dc.publisherElsevier BV
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0165783618301589
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Fisheries Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Fisheries Research, [, , (2018-06-01)] DOI: 10.1016/j.fishres.2018.05.022 . © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCoral trout
dc.subjectHybridization
dc.subjectDiagnostic markers
dc.subjectMolecular identification
dc.titleSpecies delineation and hybrid identification using diagnostic nuclear markers for Plectropomus leopardus and Plectropomus maculatus
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentMarine Science Program
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.identifier.journalFisheries Research
dc.eprint.versionPost-print
dc.contributor.institutionState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, Fujian Province, China
dc.contributor.institutionAustralian Research Council Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, QLD, 4811, Australia
kaust.personHe, Song
kaust.personBerumen, Michael L.


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NOTICE: this is the author’s version of a work that was accepted for publication in Fisheries Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Fisheries Research, [, , (2018-06-01)] DOI: 10.1016/j.fishres.2018.05.022 . © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Except where otherwise noted, this item's license is described as NOTICE: this is the author’s version of a work that was accepted for publication in Fisheries Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Fisheries Research, [, , (2018-06-01)] DOI: 10.1016/j.fishres.2018.05.022 . © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/