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dc.contributor.authorJumat, Muhammad
dc.contributor.authorHaroon, Muhammad
dc.contributor.authorAljassim, Nada I.
dc.contributor.authorCheng, Hong
dc.contributor.authorHong, Pei-Ying
dc.date.accessioned2018-05-31T06:38:46Z
dc.date.available2018-05-31T06:38:46Z
dc.date.issued2018-04-06
dc.identifier.citationJumat M, Haroon M, Al-Jassim N, Cheng H, Hong P-Y (2018) An Increase of Abundance and Transcriptional Activity for Acinetobacter junii Post Wastewater Treatment. Water 10: 436. Available: http://dx.doi.org/10.3390/w10040436.
dc.identifier.issn2073-4441
dc.identifier.doi10.3390/w10040436
dc.identifier.urihttp://hdl.handle.net/10754/628001
dc.description.abstractA membrane bioreactor (MBR)-based wastewater treatment plant (WWTP) in Saudi Arabia is assessed over a five-month period in 2015 and once in 2017 for bacterial diversity and transcriptional activity using metagenomics, metatranscriptomics and real time quantitative polymerase chain reaction (RT-qPCR). Acinetobacter spp. are shown to be enriched in the chlorinated effluent. Members of the Acinetobacter genus are the most abundant in the effluent and chlorinated effluent. At the species level, Acinetobacter junii have higher relative abundances post MBR and chlorination. RNA-seq analysis show that, in A. junii, 288 genes and 378 genes are significantly upregulated in the effluent and chlorinated effluent, respectively, with 98 genes being upregulated in both. RT-qPCR of samples in 2015 and 2017 confirm the upregulation observed in RNA-seq. Analysis of the 98 genes show that majority of the upregulated genes are involved in cellular repair and metabolism followed by resistance, virulence, and signaling. Additionally, two different subpopulations of A. junii are observed in the effluent and chlorinated effluent. The upregulation of cellular repair and metabolism genes, and the formation of different subpopulations of A. junii in both effluents provide insights into the mechanisms employed by A. junii to persist in the conditions of a WWTP.
dc.description.sponsorshipThe authors would like to thank George Princeton Dunsford for access to the KAUST wastewater treatment plant and Moustapha Harb for providing sampling assistance. The research reported in this publication was supported by the KAUST baseline funding BAS/1/1033-01-01 awarded to Pei-Ying Hong.
dc.publisherMDPI AG
dc.relation.urlhttp://www.mdpi.com/2073-4441/10/4/436
dc.rightsArchived with thanks to Water
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAcinetobacter junii
dc.subjectAntibiotic resistance
dc.subjectMetal resistance genes
dc.subjectPersistence
dc.subjectWastewater treatment plant
dc.titleAn Increase of Abundance and Transcriptional Activity for Acinetobacter junii Post Wastewater Treatment
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentEnvironmental Science and Engineering Program
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)
dc.identifier.journalWater
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, 02138, , United States
kaust.personJumat, Mohammad Raihan
kaust.personAljassim, Nada I.
kaust.personCheng, Hong
kaust.personHong, Pei-Ying
kaust.grant.numberBAS/1/1033-01-01
refterms.dateFOA2018-06-14T06:41:16Z


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