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    Genetic Determinants Associated With in Vivo Survival of Burkholderia cenocepacia in the Caenorhabditis elegans Model

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    Type
    Article
    Authors
    Wong, Yee-Chin
    Abd El Ghany, Moataz
    Naeem, Raeece cc
    Yap, Soon-Joo
    Hoh, Chee-Choong
    Pain, Arnab cc
    Nathan, Sheila
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Computational Bioscience Research Center (CBRC)
    KAUST Grant Number
    BAS/1/1020-01-01
    Date
    2018-05-29
    Permanent link to this record
    http://hdl.handle.net/10754/627990
    
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    Abstract
    A Burkholderia cenocepacia infection usually leads to reduced survival and fatal cepacia syndrome in cystic fibrosis patients. The identification of B. cenocepacia essential genes for in vivo survival is key to designing new anti-infectives therapies. We used the Transposon-Directed Insertion Sequencing (TraDIS) approach to identify genes required for B. cenocepacia survival in the model infection host, Caenorhabditis elegans. A B. cenocepacia J2315 transposon pool of ∼500,000 mutants was used to infect C. elegans. We identified 178 genes as crucial for B. cenocepacia survival in the infected nematode. The majority of these genes code for proteins of unknown function, many of which are encoded by the genomic island BcenGI13, while other gene products are involved in nutrient acquisition, general stress responses and LPS O-antigen biosynthesis. Deletion of the glycosyltransferase gene wbxB and a histone-like nucleoid structuring (H-NS) protein-encoding gene (BCAL0154) reduced bacterial accumulation and attenuated virulence in C. elegans. Further analysis using quantitative RT-PCR indicated that BCAL0154 modulates B. cenocepacia pathogenesis via transcriptional regulation of motility-associated genes including fliC, fliG, flhD, and cheB1. This screen has successfully identified genes required for B. cenocepacia survival within the host-associated environment, many of which are potential targets for developing new antimicrobials.
    Citation
    Wong Y-C, Abd El Ghany M, Ghazzali RNM, Yap S-J, Hoh C-C, et al. (2018) Genetic Determinants Associated With in Vivo Survival of Burkholderia cenocepacia in the Caenorhabditis elegans Model. Frontiers in Microbiology 9. Available: http://dx.doi.org/10.3389/fmicb.2018.01118.
    Sponsors
    This study was supported by the grants 06-05-16-MB003 from the Ministry of Science, Technology and Innovation Malaysia and DIP-2015-022 from Universiti Kebangsaan Malaysia awarded to SN. Y-CW was supported by a MyBrain15 Scholarship from the Ministry of Higher Education Malaysia. Part of this work was conducted in KAUST, funded by the faculty baseline fund BAS/1/1020-01-01 from KAUST to AP. We thank Prof. Herbert P. Schweizer (University of Florida, United States) for kindly providing the plasmid pEXKm5 and Dr. Chantratita Narisara (Mahidol University, Thailand) for her expert advice on mutant construction. We also thank Dr. Rui-Rui Wong for her technical assistance and and Ms. Jia-Shiun Khoo (Codon Genomics) for bioinformatics support.
    Publisher
    Frontiers Media SA
    Journal
    Frontiers in Microbiology
    DOI
    10.3389/fmicb.2018.01118
    Additional Links
    https://www.frontiersin.org/articles/10.3389/fmicb.2018.01118/full
    ae974a485f413a2113503eed53cd6c53
    10.3389/fmicb.2018.01118
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Computational Bioscience Research Center (CBRC)

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