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dc.contributor.authorVigani, Gianpiero
dc.contributor.authorRolli, Eleonora
dc.contributor.authorMarasco, Ramona
dc.contributor.authorDell'Orto, Marta
dc.contributor.authorMichoud, Gregoire
dc.contributor.authorSoussi, Asma
dc.contributor.authorRaddadi, Noura
dc.contributor.authorBorin, Sara
dc.contributor.authorSorlini, Claudia
dc.contributor.authorZocchi, Graziano
dc.contributor.authorDaffonchio, Daniele
dc.date.accessioned2018-05-24T10:35:28Z
dc.date.available2018-05-24T10:35:28Z
dc.date.issued2018-05-22
dc.identifier.citationVigani G, Rolli E, Marasco R, Dell’Orto M, Michoud G, et al. (2018) Root bacterial endophytes confer drought resistance and enhance expression and activity of a vacuolar H+ -pumping pyrophosphatase in pepper plants. Environmental Microbiology. Available: http://dx.doi.org/10.1111/1462-2920.14272.
dc.identifier.issn1462-2912
dc.identifier.doi10.1111/1462-2920.14272
dc.identifier.urihttp://hdl.handle.net/10754/627951
dc.description.abstractIt has been previously shown that the transgenic overexpression of the plant root vacuolar proton pumps H+ -ATPase (V-ATPase) and H+ -PPase (V-PPase) confer tolerance to drought. Since plant-root endophytic bacteria can also promote drought tolerance, we hypothesize that such promotion can be associated to the enhancement of the host vacuolar proton pumps expression and activity. To test this hypothesis, we selected two endophytic bacteria endowed with an array of in vitro plant growth promoting traits. Their genome sequences confirmed the presence of traits previously shown to confer drought resistance to plants, such as the synthesis of nitric oxide and of organic volatile organic compounds. We used the two strains on pepper (Capsicuum annuum L.) because of its high sensitivity to drought. Under drought conditions, both strains stimulated a larger root system and enhanced the leaves' photosynthetic activity. By testing the expression and activity of the vacuolar proton pumps, H+ -ATPase (V-ATPase) and H+ -PPase (V-PPase), we found that bacterial colonization enhanced V-PPase only. We conclude that the enhanced expression and activity of V-PPase can be favoured by the colonization of drought-tolerance-inducing bacterial endophytes. This article is protected by copyright. All rights reserved.
dc.description.sponsorshipThe authors declare an absence of competing financial interests. This research was supported by funding from the Italian MIUR FIRB project no. RBIN047MBH “Strategy to improve crop productivity under water stress”, the EU project BIODESERT (European Community’s Seventh Framework Programme CSA-SA REGPOT-2008-2) under grant agreement no. 245746, and King Abdullah University of Science and Technology (KAUST) baseline research funds to DD. ER acknowledges support from Università degli Studi di Milano, DeFENS, Regione Lombardia (contract ‘Dote Ricerca’). The authors thank (i) Dr. Vasco Meneghini for support with the confocal microscopy analysis and interpretation, and a critical reading of the manuscript; (ii) Dr. Umberto Fascio at the Centro Interdipartimentale di Microscopia Applicata of the University of Milan for technical support with the confocal microscope; (iii) Dr. M. Maeshima of Nagoya University, Nagoya, Japan, for the kind gift of antibodies against V-ATPase and V-PPase; (iv) Dr. Bessem Chouaia of the University of Milan for the help in the bacterial genome assembly and (v) Dr. Alessia Perego and Dr. Patrizia Zaccheo of the University of Milan and (vi) Dr. Marco Fusi of King Abdullah University of Science and Technology for assistance with the statistical analysis; (vii) Francesco Della Valle of King Abdullah University of Science and Technology for help in critical revision of the manuscript. The authors declare that they have no conflict of interest.
dc.publisherWiley
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.14272
dc.rightsArchived with thanks to Environmental Microbiology
dc.subjectDrought stress
dc.subjectPepper
dc.subjectEndophytes
dc.subjectPlant-microbe Interaction
dc.subjectPlant Growth Promoting Bacteria
dc.subjectV-ppase
dc.subjectBeneficial Microbes
dc.subjectVacuolar Proton Pumps
dc.titleRoot bacterial endophytes confer drought resistance and enhance expression and activity of a vacuolar H+ -pumping pyrophosphatase in pepper plants
dc.typeArticle
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentEnvironmental Science and Engineering Program
dc.contributor.departmentBioscience Program
dc.identifier.journalEnvironmental Microbiology
dc.eprint.versionPost-print
dc.contributor.institutionUniversity of Turin, Plant Physiology Unit, Department of Life Sciences and Systems Biology; 10135 Turin Italy
dc.contributor.institutionUniversity of Milan, Department of Food, Environmental and Nutritional Sciences (DeFENS); 20133 Milan Italy
dc.contributor.institutionUniversity of Milan, Department of Agricultural and Environmental Sciences, Production, Landscape, Agroenergy (DISAA); 20133 Milan Italy
dc.contributor.institutionAlma Mater Studiorum University of Bologna, Department of Civil, Chemical, Environmental and Materials Engineering (DICAM); Bologna Italy
kaust.personMarasco, Ramona
kaust.personMichoud, Gregoire
kaust.personSoussi, Asma
kaust.personDaffonchio, Daniele


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