Poly(3-hydroxybutyrate) production in an integrated electromicrobial setup: Investigation under stress-inducing conditions
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Type
ArticleAuthors
Al Rowaihi, Israa
Paillier, Alexis
Rasul, Shahid

Karan, Ram
Grötzinger, Stefan Wolfgang
Takanabe, Kazuhiro

Eppinger, Jörg

KAUST Department
Biological & Organometallic Catalysis LaboratoriesBiological and Environmental Sciences and Engineering (BESE) Division
Bioscience Program
Catalysis for Energy Conversion (CatEC)
Chemical Science Program
KAUST Catalysis Center (KCC)
Office of the VP
Physical Science and Engineering (PSE) Division
Water Desalination and Reuse Research Center (WDRC)
Date
2018-04-26Permanent link to this record
http://hdl.handle.net/10754/627694
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Show full item recordAbstract
Poly(3-hydroxybutyrate) (PHB), a biodegradable polymer, can be produced by different microorganisms. The PHB belongs to the family of polyhydroxyalkanoate (PHA) that mostly accumulates as a granule in the cytoplasm of microorganisms to store carbon and energy. In this study, we established an integrated one-pot electromicrobial setup in which carbon dioxide is reduced to formate electrochemically, followed by sequential microbial conversion into PHB, using the two model strains, Methylobacterium extorquens AM1 and Cupriavidus necator H16. This setup allows to investigate the influence of different stress conditions, such as coexisting electrolysis, relatively high salinity, nutrient limitation, and starvation, on the production of PHB. The overall PHB production efficiency was analyzed in reasonably short reaction cycles typically as short as 8 h. As a result, the PHB formation was detected with C. necator H16 as a biocatalyst only when the electrolysis was operated in the same solution. The specificity of the source of PHB production is discussed, such as salinity, electricity, concurrent hydrogen production, and the possible involvement of reactive oxygen species (ROS).Citation
Al Rowaihi IS, Paillier A, Rasul S, Karan R, Grötzinger SW, et al. (2018) Poly(3-hydroxybutyrate) production in an integrated electromicrobial setup: Investigation under stress-inducing conditions. PLOS ONE 13: e0196079. Available: http://dx.doi.org/10.1371/journal.pone.0196079.Sponsors
The research reported in this publication was supported by King Abdullah University of Science and Technology (KAUST).Publisher
Public Library of Science (PLoS)Journal
PLOS ONEPubMed ID
29698424ae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0196079
Scopus Count
Collections
Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Bioscience Program; Physical Science and Engineering (PSE) Division; Chemical Science Program; KAUST Catalysis Center (KCC); KAUST Catalysis Center (KCC); Water Desalination and Reuse Research Center (WDRC); Water Desalination and Reuse Research Center (WDRC)
Except where otherwise noted, this item's license is described as This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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