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dc.contributor.authorMauriello Jimenez, Chiara
dc.contributor.authorAggad, Dina
dc.contributor.authorCroissant, Jonas G.
dc.contributor.authorTresfield, Karen
dc.contributor.authorLaurencin, Danielle
dc.contributor.authorBerthomieu, Dorothée
dc.contributor.authorCubedo, Nicolas
dc.contributor.authorRossel, Mireille
dc.contributor.authorAlsaiari, Shahad K.
dc.contributor.authorAnjum, Dalaver H.
dc.contributor.authorSougrat, Rachid
dc.contributor.authorRoldan-Gutierrez, Manuel
dc.contributor.authorRicheter, Sébastien
dc.contributor.authorOliviero, Erwan
dc.contributor.authorRaehm, Laurence
dc.contributor.authorCharnay, Clarence
dc.contributor.authorCattoën, Xavier
dc.contributor.authorClément, Sébastien
dc.contributor.authorWong Chi Man, Michel
dc.contributor.authorMaynadier, Marie
dc.contributor.authorChaleix, Vincent
dc.contributor.authorSol, Vincent
dc.contributor.authorGarcia, Marcel
dc.contributor.authorGary-Bobo, Magali
dc.contributor.authorKhashab, Niveen M.
dc.contributor.authorBettache, Nadir
dc.contributor.authorDurand, Jean-Olivier
dc.date.accessioned2018-04-24T07:48:29Z
dc.date.available2018-04-24T07:48:29Z
dc.date.issued2018-03-30
dc.identifier.citationMauriello Jimenez C, Aggad D, Croissant JG, Tresfield K, Laurencin D, et al. (2018) Porous Porphyrin-Based Organosilica Nanoparticles for NIR Two-Photon Photodynamic Therapy and Gene Delivery in Zebrafish. Advanced Functional Materials: 1800235. Available: http://dx.doi.org/10.1002/adfm.201800235.
dc.identifier.issn1616-301X
dc.identifier.doi10.1002/adfm.201800235
dc.identifier.doi10.1002/adfm.201870143
dc.identifier.urihttp://hdl.handle.net/10754/627633
dc.description.abstractPeriodic mesoporous organosilica nanoparticles emerge as promising vectors for nanomedicine applications. Their properties are very different from those of well-known mesoporous silica nanoparticles as there is no silica source for their synthesis. So far, they have only been synthesized from small bis-silylated organic precursors. However, no studies employing large stimuli-responsive precursors have been reported on such hybrid systems yet. Here, the synthesis of porphyrin-based organosilica nanoparticles from a large octasilylated metalated porphyrin precursor is described for applications in near-infrared two-photon-triggered spatiotemporal theranostics. The nanoparticles display unique interconnected large cavities of 10-80 nm. The framework of the nanoparticles is constituted with J-aggregates of porphyrins, which endows them with two-photon sensitivity. The nanoparticle efficiency for intracellular tracking is first demonstrated by the in vitro near-infrared imaging of breast cancer cells. After functionalization of the nanoparticles with aminopropyltriethoxysilane, two-photon-excited photodynamic therapy in zebrafish is successfully achieved. Two-photon photochemical internalization in cancer cells of the nanoparticles loaded with siRNA is also performed for the first time. Furthermore, siRNA targeting green fluorescent protein complexed with the nanoparticles is delivered in vivo in zebrafish embryos, which demonstrates the versatility of the nanovectors for biomedical applications.
dc.description.sponsorshipC.M.J. and D.A. contributed equally to this work. The grant “Chercheur d'Avenir Languedoc-Roussillon” attributed to M.G.-B. and the ANR nanoptPDT are gratefully acknowledged. The authors thank L. Lichon for technical assistance. DFT calculations were performed using HPC resources from the CINES (projects x2016087394 and x2015087394), and from the CALMIP (Grant 2016-[P16042]). The authors thank MRI (Montpellier RIO Imaging platform) for confocal and multiphoton imaging facilities.
dc.publisherWiley
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201800235
dc.subjectNucleic acid delivery
dc.subjectOrganosilica nanoparticles
dc.subjectPorphyrins
dc.subjectTwo-photon photodynamic therapy
dc.subjectZebrafish embryos
dc.titlePorous Porphyrin-Based Organosilica Nanoparticles for NIR Two-Photon Photodynamic Therapy and Gene Delivery in Zebrafish
dc.typeArticle
dc.contributor.departmentAdvanced Membranes and Porous Materials Research Center
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentChemical Science Program
dc.contributor.departmentElectron Microscopy
dc.contributor.departmentImaging and Characterization Core Lab
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.contributor.departmentSmart Hybrid Materials (SHMs) lab
dc.identifier.journalAdvanced Functional Materials
dc.contributor.institutionInstitut Charles Gerhardt Montpellier; UMR-5253 CNRS-UM-ENSCM cc 1701; Place Eugène Bataillon 34095 Montpellier cedex 05 France
dc.contributor.institutionInstitut des Biomolécules Max Mousseron; UMR 5247 CNRS; UM-Faculté de Pharmacie; 15 Avenue Charles Flahault 34093 Montpellier cedex 05 France
dc.contributor.institutionINSERM U1198; MMDN; Université Montpellier; cc 105, Place Eugène Bataillon 34095 Montpellier cedex 05 France
dc.contributor.institutionInstitut Néel; CNRS and Université Grenoble-Alpes; 38042 Grenoble France
dc.contributor.institutionNanoMedSyn; 15 Avenue Charles Flahault 34000 Montpellier cedex 05 France
dc.contributor.institutionLaboratoire PEIRENE, EA 7500 Université de Limoges; 87060 Limoges France
kaust.personCroissant, Jonas G.
kaust.personAlsaiari, Shahad K.
kaust.personAnjum, Dalaver H.
kaust.personSougrat, Rachid
kaust.personRoldan-Gutierrez, Manuel
kaust.personKhashab, Niveen M.
dc.date.published-online2018-03-30
dc.date.published-print2018-05


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