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dc.contributor.authorCziesielski, Maha Joana
dc.contributor.authorLiew, Yi Jin
dc.contributor.authorCui, Guoxin
dc.contributor.authorSchmidt-Roach, Sebastian
dc.contributor.authorCampana, Sara
dc.contributor.authorMarondedze, Claudius
dc.contributor.authorAranda, Manuel
dc.date.accessioned2018-04-24T06:46:18Z
dc.date.available2018-04-24T06:46:18Z
dc.date.issued2018-04-18
dc.identifier.citationCziesielski MJ, Liew YJ, Cui G, Schmidt-Roach S, Campana S, et al. (2018) Multi-omics analysis of thermal stress response in a zooxanthellate cnidarian reveals the importance of associating with thermotolerant symbionts. Proceedings of the Royal Society B: Biological Sciences 285: 20172654. Available: http://dx.doi.org/10.1098/rspb.2017.2654.
dc.identifier.issn0962-8452
dc.identifier.issn1471-2954
dc.identifier.pmid29669898
dc.identifier.doi10.1098/rspb.2017.2654
dc.identifier.urihttp://hdl.handle.net/10754/627604
dc.description.abstractCorals and their endosymbiotic dinoflagellates of the genus Symbiodinium have a fragile relationship that breaks down under heat stress, an event known as bleaching. However, many coral species have adapted to high temperature environments such as the Red Sea (RS). To investigate mechanisms underlying temperature adaptation in zooxanthellate cnidarians we compared transcriptome- and proteome-wide heat stress response (24 h at 32°C) of three strains of the model organism Aiptasia pallida from regions with differing temperature profiles; North Carolina (CC7), Hawaii (H2) and the RS. Correlations between transcript and protein levels were generally low but inter-strain comparisons highlighted a common core cnidarian response to heat stress, including protein folding and oxidative stress pathways. RS anemones showed the strongest increase in antioxidant gene expression and exhibited significantly lower reactive oxygen species (ROS) levels in hospite However, comparisons of antioxidant gene and protein expression between strains did not show strong differences, indicating similar antioxidant capacity across the strains. Subsequent analysis of ROS production in isolated symbionts confirmed that the observed differences of ROS levels in hospite were symbiont-driven. Our findings indicate that RS anemones do not show increased antioxidant capacity but may have adapted to higher temperatures through association with more thermally tolerant symbionts.
dc.description.sponsorshipResearch reported in this publication was supported by funding from KAUST. We thank Craig Michell for RNAseq library preparation, the KAUST Bioscience Core Laboratory for library sequencing, Huoming Zhang for help in protein sequencing, and Marcela Herrera for collecting Aiptasia from the Red Sea.
dc.publisherThe Royal Society
dc.relation.urlhttp://rspb.royalsocietypublishing.org/content/285/1877/20172654
dc.rights© 2018 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectOxidative stress
dc.subjectHeat stress
dc.subjectProteomics
dc.subjectthermotolerance
dc.subjectTranscriptomics
dc.subjectAiptasia
dc.titleMulti-omics analysis of thermal stress response in a zooxanthellate cnidarian reveals the importance of associating with thermotolerant symbionts
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentMarine Science Program
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.identifier.journalProceedings of the Royal Society B: Biological Sciences
dc.eprint.versionPublisher's Version/PDF
kaust.personCziesielski Olschowsky, Maha Joana
kaust.personLiew, Yi Jin
kaust.personCui, Guoxin
kaust.personSchmidt-Roach, Sebastian
kaust.personCampana, Sara
kaust.personMarondedze, Claudius
kaust.personAranda, Manuel
refterms.dateFOA2018-06-14T04:24:54Z
display.relations<b> Is Supplemented By:</b> <br/> <ul> <li><i>[Dataset]</i> <br/> . DOI: <a href="https://doi.org/10.6084/m9.figshare.c.4050773">10.6084/m9.figshare.c.4050773</a> HANDLE: <a href="http://hdl.handle.net/10754/664067">10754/664067</a></li></ul>
dc.date.published-online2018-04-18
dc.date.published-print2018-04-25


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© 2018 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as © 2018 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.