Multi-omics analysis of thermal stress response in a zooxanthellate cnidarian reveals the importance of associating with thermotolerant symbionts
Type
ArticleAuthors
Cziesielski, Maha Joana
Liew, Yi Jin

Cui, Guoxin

Schmidt-Roach, Sebastian
Campana, Sara
Marondedze, Claudius

Aranda, Manuel

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Marine Science Program
Red Sea Research Center (RSRC)
Date
2018-04-18Online Publication Date
2018-04-18Print Publication Date
2018-04-25Permanent link to this record
http://hdl.handle.net/10754/627604
Metadata
Show full item recordAbstract
Corals and their endosymbiotic dinoflagellates of the genus Symbiodinium have a fragile relationship that breaks down under heat stress, an event known as bleaching. However, many coral species have adapted to high temperature environments such as the Red Sea (RS). To investigate mechanisms underlying temperature adaptation in zooxanthellate cnidarians we compared transcriptome- and proteome-wide heat stress response (24 h at 32°C) of three strains of the model organism Aiptasia pallida from regions with differing temperature profiles; North Carolina (CC7), Hawaii (H2) and the RS. Correlations between transcript and protein levels were generally low but inter-strain comparisons highlighted a common core cnidarian response to heat stress, including protein folding and oxidative stress pathways. RS anemones showed the strongest increase in antioxidant gene expression and exhibited significantly lower reactive oxygen species (ROS) levels in hospite However, comparisons of antioxidant gene and protein expression between strains did not show strong differences, indicating similar antioxidant capacity across the strains. Subsequent analysis of ROS production in isolated symbionts confirmed that the observed differences of ROS levels in hospite were symbiont-driven. Our findings indicate that RS anemones do not show increased antioxidant capacity but may have adapted to higher temperatures through association with more thermally tolerant symbionts.Citation
Cziesielski MJ, Liew YJ, Cui G, Schmidt-Roach S, Campana S, et al. (2018) Multi-omics analysis of thermal stress response in a zooxanthellate cnidarian reveals the importance of associating with thermotolerant symbionts. Proceedings of the Royal Society B: Biological Sciences 285: 20172654. Available: http://dx.doi.org/10.1098/rspb.2017.2654.Sponsors
Research reported in this publication was supported by funding from KAUST. We thank Craig Michell for RNAseq library preparation, the KAUST Bioscience Core Laboratory for library sequencing, Huoming Zhang for help in protein sequencing, and Marcela Herrera for collecting Aiptasia from the Red Sea.Publisher
The Royal SocietyPubMed ID
29669898Additional Links
http://rspb.royalsocietypublishing.org/content/285/1877/20172654Relations
Is Supplemented By:- [Dataset]
Cziesielski, M. J., Liew, Y. J., Guoxin Cui, Schmidt-Roach, S., Campana, S., Marondedze, C., & Aranda, M. (2018). Supplementary material from "Multi-omics analysis of thermal stress response in a zooxanthellate cnidarian reveals the importance of associating with thermotolerant symbionts". Figshare. https://doi.org/10.6084/M9.FIGSHARE.C.4050773. DOI: 10.6084/m9.figshare.c.4050773 Handle: 10754/664067
ae974a485f413a2113503eed53cd6c53
10.1098/rspb.2017.2654
Scopus Count
Except where otherwise noted, this item's license is described as © 2018 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
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