Multi-omics analysis of thermal stress response in a zooxanthellate cnidarian reveals the importance of associating with thermotolerant symbionts
AuthorsCziesielski, Maha J.
Liew, Yi Jin
KAUST DepartmentRed Sea Research Center (RSRC)
Biological and Environmental Sciences and Engineering (BESE) Division
Marine Science Program
Permanent link to this recordhttp://hdl.handle.net/10754/627604
MetadataShow full item record
AbstractCorals and their endosymbiotic dinoflagellates of the genus Symbiodinium have a fragile relationship that breaks down under heat stress, an event known as bleaching. However, many coral species have adapted to high temperature environments such as the Red Sea (RS). To investigate mechanisms underlying temperature adaptation in zooxanthellate cnidarians we compared transcriptome- and proteome-wide heat stress response (24 h at 32°C) of three strains of the model organism Aiptasia pallida from regions with differing temperature profiles; North Carolina (CC7), Hawaii (H2) and the RS. Correlations between transcript and protein levels were generally low but inter-strain comparisons highlighted a common core cnidarian response to heat stress, including protein folding and oxidative stress pathways. RS anemones showed the strongest increase in antioxidant gene expression and exhibited significantly lower reactive oxygen species (ROS) levels in hospite However, comparisons of antioxidant gene and protein expression between strains did not show strong differences, indicating similar antioxidant capacity across the strains. Subsequent analysis of ROS production in isolated symbionts confirmed that the observed differences of ROS levels in hospite were symbiont-driven. Our findings indicate that RS anemones do not show increased antioxidant capacity but may have adapted to higher temperatures through association with more thermally tolerant symbionts.
CitationCziesielski MJ, Liew YJ, Cui G, Schmidt-Roach S, Campana S, et al. (2018) Multi-omics analysis of thermal stress response in a zooxanthellate cnidarian reveals the importance of associating with thermotolerant symbionts. Proceedings of the Royal Society B: Biological Sciences 285: 20172654. Available: http://dx.doi.org/10.1098/rspb.2017.2654.
SponsorsResearch reported in this publication was supported by funding from KAUST. We thank Craig Michell for RNAseq library preparation, the KAUST Bioscience Core Laboratory for library sequencing, Huoming Zhang for help in protein sequencing, and Marcela Herrera for collecting Aiptasia from the Red Sea.
PublisherThe Royal Society
Except where otherwise noted, this item's license is described as © 2018 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
- Summarized datasheet for multi-omics response of three Exaiptasia strains to heat stress: a new way to process omics data.
- Authors: Cziesielski MJ, Liew YJ, Aranda M
- Issue date: 2018 Dec 18
- Optimal nutrient exchange and immune responses operate in partner specificity in the cnidarian-dinoflagellate symbiosis.
- Authors: Matthews JL, Crowder CM, Oakley CA, Lutz A, Roessner U, Meyer E, Grossman AR, Weis VM, Davy SK
- Issue date: 2017 Dec 12
- Symbiosis induces widespread changes in the proteome of the model cnidarian Aiptasia.
- Authors: Oakley CA, Ameismeier MF, Peng L, Weis VM, Grossman AR, Davy SK
- Issue date: 2016 Jul
- Change in algal symbiont communities after bleaching, not prior heat exposure, increases heat tolerance of reef corals.
- Authors: Silverstein RN, Cunning R, Baker AC
- Issue date: 2015 Jan
- Warm preconditioning protects against acute heat-induced respiratory dysfunction and delays bleaching in a symbiotic sea anemone.
- Authors: Hawkins TD, Warner ME
- Issue date: 2017 Mar 15