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dc.contributor.authorChauhan, Sushma
dc.contributor.authorRahman, Hifzur
dc.contributor.authorMastan, Shaik G.
dc.contributor.authorSudheer, Pamidimarri D.V.N.
dc.contributor.authorReddy, Muppala P.
dc.date.accessioned2018-04-24T06:46:17Z
dc.date.available2018-04-24T06:46:17Z
dc.date.issued2018-04-22
dc.identifier.citationChauhan S, Rahman H, Mastan SG, Sudheer PDVN, Reddy MP (2018) Isolation and sequence characterization of DNA-A genome of a new begomovirus strain associated with severe leaf curling symptoms of Jatropha curcas L. Gene. Available: http://dx.doi.org/10.1016/j.gene.2018.04.062.
dc.identifier.issn0378-1119
dc.identifier.pmid29684487
dc.identifier.doi10.1016/j.gene.2018.04.062
dc.identifier.urihttp://hdl.handle.net/10754/627603
dc.description.abstractBegomoviruses belong to the family Geminiviridae are associated with several disease symptoms, such as mosaic and leaf curling in Jatropha curcas. The molecular characterization of these viral strains will help in developing management strategies to control the disease. In this study, J. curcas that was infected with begomovirus and showed acute leaf curling symptoms were identified. DNA-A segment from pathogenic viral strain was isolated and sequenced. The sequenced genome was assembled and characterized in detail. The full-length DNA-A sequence was covered by primer walking. The genome sequence showed the general organization of DNA-A from begomovirus by the distribution of ORFs in both viral and anti-viral strands. The genome size ranged from 2844 bp–2852 bp. Three strains with minor nucleotide variations were identified, and a phylogenetic analysis was performed by comparing the DNA-A segments from other reported begomovirus isolates. The maximum sequence similarity was observed with Euphorbia yellow mosaic virus (FN435995). In the phylogenetic tree, no clustering was observed with previously reported begomovirus strains isolated from J. curcas host. The strains isolated in this study belong to new begomoviral strain that elicits symptoms of leaf curling in J. curcas. The results indicate that the probable origin of the strains is from Jatropha mosaic virus infecting J. gassypifolia. The strains isolated in this study are referred as Jatropha curcas leaf curl India virus (JCLCIV) based on the major symptoms exhibited by host J. curcas.
dc.description.sponsorshipWe are thankful to the Council for Scientific and Industrial Research, New Delhi, India for its financial support for the SRF and research associate fellowships.
dc.publisherElsevier BV
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0378111918304360
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Gene. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Gene, 22 April 2018. DOI: 10.1016/j.gene.2018.04.062. © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBegomovirus
dc.subjectBiodiesel
dc.subjectJatropha curcas leaf curl India virus (JCLCV)
dc.subjectJatropha curcas L.
dc.titleIsolation and sequence characterization of DNA-A genome of a new begomovirus strain associated with severe leaf curling symptoms of Jatropha curcas L.
dc.typeArticle
dc.contributor.departmentGreenhouse Core Lab
dc.contributor.departmentPlant Stress Genomics Research Lab
dc.identifier.journalGene
dc.eprint.versionPost-print
dc.contributor.institutionDepartment of Chemical and Biochemical Engineering, Dongguk University–Seoul, Seoul 100-715, Republic of Korea
dc.contributor.institutionDepartment of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003, India
dc.contributor.institutionDepartment of Biotechnology, Aditya Degree and PG college, Adikavinannaya University, Laxminarayana Nagar, Kakinada 533003, Andhra Pradesh, India
dc.contributor.institutionDepartment of Environmental Engineering, College of Engineering, Ajou University, Suwon, Gyeonggi-do, Republic of Korea
kaust.personReddy, Muppala P.
refterms.dateFOA2019-04-22T00:00:00Z
dc.date.published-online2018-04-22
dc.date.published-print2018-07


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