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    Isolation and sequence characterization of DNA-A genome of a new begomovirus strain associated with severe leaf curling symptoms of Jatropha curcas L.

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    Type
    Article
    Authors
    Chauhan, Sushma
    Rahman, Hifzur
    Mastan, Shaik G.
    Sudheer, Pamidimarri D.V.N.
    Reddy, Muppala P.
    KAUST Department
    Greenhouse Core Lab
    Plant Stress Genomics Research Lab
    Date
    2018-04-22
    Online Publication Date
    2018-04-22
    Print Publication Date
    2018-07
    Permanent link to this record
    http://hdl.handle.net/10754/627603
    
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    Abstract
    Begomoviruses belong to the family Geminiviridae are associated with several disease symptoms, such as mosaic and leaf curling in Jatropha curcas. The molecular characterization of these viral strains will help in developing management strategies to control the disease. In this study, J. curcas that was infected with begomovirus and showed acute leaf curling symptoms were identified. DNA-A segment from pathogenic viral strain was isolated and sequenced. The sequenced genome was assembled and characterized in detail. The full-length DNA-A sequence was covered by primer walking. The genome sequence showed the general organization of DNA-A from begomovirus by the distribution of ORFs in both viral and anti-viral strands. The genome size ranged from 2844 bp–2852 bp. Three strains with minor nucleotide variations were identified, and a phylogenetic analysis was performed by comparing the DNA-A segments from other reported begomovirus isolates. The maximum sequence similarity was observed with Euphorbia yellow mosaic virus (FN435995). In the phylogenetic tree, no clustering was observed with previously reported begomovirus strains isolated from J. curcas host. The strains isolated in this study belong to new begomoviral strain that elicits symptoms of leaf curling in J. curcas. The results indicate that the probable origin of the strains is from Jatropha mosaic virus infecting J. gassypifolia. The strains isolated in this study are referred as Jatropha curcas leaf curl India virus (JCLCIV) based on the major symptoms exhibited by host J. curcas.
    Citation
    Chauhan S, Rahman H, Mastan SG, Sudheer PDVN, Reddy MP (2018) Isolation and sequence characterization of DNA-A genome of a new begomovirus strain associated with severe leaf curling symptoms of Jatropha curcas L. Gene. Available: http://dx.doi.org/10.1016/j.gene.2018.04.062.
    Sponsors
    We are thankful to the Council for Scientific and Industrial Research, New Delhi, India for its financial support for the SRF and research associate fellowships.
    Publisher
    Elsevier BV
    Journal
    Gene
    DOI
    10.1016/j.gene.2018.04.062
    PubMed ID
    29684487
    Additional Links
    http://www.sciencedirect.com/science/article/pii/S0378111918304360
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.gene.2018.04.062
    Scopus Count
    Collections
    Greenhouse Core Lab; Articles

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