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dc.contributor.authorJaremko, Lukasz
dc.contributor.authorJaremko, Mariusz
dc.contributor.authorEjchart, Andrzej
dc.contributor.authorNowakowski, Michał
dc.date.accessioned2018-04-16T11:27:42Z
dc.date.available2018-04-16T11:27:42Z
dc.date.issued2018-03-28
dc.identifier.citationJaremko Ł, Jaremko M, Ejchart A, Nowakowski M (2018) Fast evaluation of protein dynamics from deficient 15N relaxation data. Journal of Biomolecular NMR. Available: http://dx.doi.org/10.1007/s10858-018-0176-3.
dc.identifier.issn0925-2738
dc.identifier.issn1573-5001
dc.identifier.pmid29594733
dc.identifier.doi10.1007/s10858-018-0176-3
dc.identifier.urihttp://hdl.handle.net/10754/627505
dc.description.abstractSimple and convenient method of protein dynamics evaluation from the insufficient experimental N relaxation data is presented basing on the ratios, products, and differences of longitudinal and transverse N relaxation rates obtained at a single magnetic field. Firstly, the proposed approach allows evaluating overall tumbling correlation time (nanosecond time scale). Next, local parameters of the model-free approach characterizing local mobility of backbone amide N–H vectors on two different time scales, S and R, can be elucidated. The generalized order parameter, S, describes motions on the time scale faster than the overall tumbling correlation time (pico- to nanoseconds), while the chemical exchange term, R, identifies processes slower than the overall tumbling correlation time (micro- to milliseconds). Advantages and disadvantages of different methods of data handling are thoroughly discussed.
dc.description.sponsorshipThe research by LJ and MJ reported in this publication was supported by funding from King Abdullah University of Science and Technology (KAUST).
dc.publisherSpringer Nature
dc.relation.urlhttp://link.springer.com/article/10.1007/s10858-018-0176-3
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject15N magnetic relaxation
dc.subjectModel-free approach
dc.subjectProtein dynamics
dc.subjectRatio, product, and difference of relaxation rates
dc.subjectSemi-quantitative analysis of 15N relaxation data
dc.titleFast evaluation of protein dynamics from deficient 15N relaxation data
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.identifier.journalJournal of Biomolecular NMR
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionInstitute of Biochemistry and Biophysics, Polish Academy of Science, Pawinskiego 5A, Warszawa, 02-106, , , Poland
dc.contributor.institutionFaculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, Warszawa, 02-089, , , Poland
kaust.personJaremko, Lukasz
kaust.personJaremko, Mariusz
refterms.dateFOA2018-06-14T04:20:23Z
dc.date.published-online2018-03-28
dc.date.published-print2018-04


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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Except where otherwise noted, this item's license is described as This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.