miR-126-5p by direct targeting of JNK-interacting protein-2 (JIP-2) plays a key role in Theileria-infected macrophage virulence
Ansari, Hifzur Rahman
Ben Rached, Fathia
Latre De Late, Perle
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Pathogen Genomics Laboratory
KAUST Grant NumberOSR-2015-CRG4-2610
MetadataShow full item record
AbstractTheileria annulata is an apicomplexan parasite that infects and transforms bovine macrophages that disseminate throughout the animal causing a leukaemia-like disease called tropical theileriosis. Using deep RNAseq of T. annulata-infected B cells and macrophages we identify a set of microRNAs induced by infection, whose expression diminishes upon loss of the hyper-disseminating phenotype of virulent transformed macrophages. We describe how infection-induced upregulation of miR-126-5p ablates JIP-2 expression to release cytosolic JNK to translocate to the nucleus and trans-activate AP-1-driven transcription of mmp9 to promote tumour dissemination. In non-disseminating attenuated macrophages miR-126-5p levels drop, JIP-2 levels increase, JNK1 is retained in the cytosol leading to decreased c-Jun phosphorylation and dampened AP-1-driven mmp9 transcription. We show that variation in miR-126-5p levels depends on the tyrosine phosphorylation status of AGO2 that is regulated by Grb2-recruitment of PTP1B. In attenuated macrophages Grb2 levels drop resulting in less PTP1B recruitment, greater AGO2 phosphorylation, less miR-126-5p associated with AGO2 and a consequent rise in JIP-2 levels. Changes in miR-126-5p levels therefore, underpin both the virulent hyper-dissemination and the attenuated dissemination of T. annulata-infected macrophages.
CitationHaidar M, Rchiad Z, Ansari HR, Ben-Rached F, Tajeri S, et al. (2018) miR-126-5p by direct targeting of JNK-interacting protein-2 (JIP-2) plays a key role in Theileria-infected macrophage virulence. PLOS Pathogens 14: e1006942. Available: http://dx.doi.org/10.1371/journal.ppat.1006942.
SponsorsThis study was supported by the Competitive Research Grant (OSR-2015-CRG4-2610) from the Office for Sponsored Research (OSR, https://osr.kaust.edu.sa/crf/Funding/Pages/CRFCRG.aspx) in King Abdullah University of Science and Technology (KAUST). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
PublisherPublic Library of Science (PLoS)
Except where otherwise noted, this item's license is described as This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Engineering attenuated virulence of a Theileria annulata-infected macrophage.
- Authors: Echebli N, Mhadhbi M, Chaussepied M, Vayssettes C, Di Santo JP, Darghouth MA, Langsley G
- Issue date: 2014
- TGF-β2 induces Grb2 to recruit PI3-K to TGF-RII that activates JNK/AP-1-signaling and augments invasiveness of Theileria-transformed macrophages.
- Authors: Haidar M, Whitworth J, Noé G, Liu WQ, Vidal M, Langsley G
- Issue date: 2015 Oct 29
- Infection with Theileria annulata induces expression of matrix metalloproteinase 9 and transcription factor AP-1 in bovine leucocytes.
- Authors: Baylis HA, Megson A, Hall R
- Issue date: 1995 Feb
- Filopodia and membrane blebs drive efficient matrix invasion of macrophages transformed by the intracellular parasite Theileria annulata.
- Authors: Ma M, Baumgartner M
- Issue date: 2013
- The protozoan parasite Theileria annulata alters the differentiation state of the infected macrophage and suppresses musculoaponeurotic fibrosarcoma oncogene (MAF) transcription factors.
- Authors: Jensen K, Makins GD, Kaliszewska A, Hulme MJ, Paxton E, Glass EJ
- Issue date: 2009 Aug