Effect of Salt on the Metabolism of ‘Candidatus Accumulibacter’ Clade I and II
Type
ArticleKAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionEnvironmental Science and Engineering Program
Marine Science Program
Water Desalination and Reuse Research Center (WDRC)
Date
2018-03-16Permanent link to this record
http://hdl.handle.net/10754/627386
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Saline wastewater is known to affect the performance of phosphate-accumulating organisms (PAOs) in enhanced biological phosphorus removal (EBPR) process. However, studies comparing the effect of salinity on different PAO clades are lacking. In this study, 'Candidatus Accumulibacter phosphatis' Clade I and II (hereafter referred to as PAOI and PAOII) were highly enriched (~90% in relative abundance as determined by quantitative FISH) in the form of granules in two sequencing batch reactors. Anaerobic and aerobic batch experiments were conducted to evaluate the effect of salinity on the kinetics and stoichiometry of PAOI and PAOII. PAOI and PAOII communities showed different priority in using polyphosphate (poly-P) and glycogen to generate ATP in the anaerobic phase when exposed to salt, with PAOI depending more on intracellular poly-P degradation (e.g., the proportion of calculated ATP derived from poly-P increased by 5-6% at 0.256 mol/L NaCl or KCl) while PAOII on glycolysis of intracellularly stored glycogen (e.g., the proportion of calculated ATP derived from glycogen increased by 29-30% at 0.256 mol/L NaCl or KCl). In the aerobic phase, the loss of phosphate uptake capability was more pronounced in PAOII due to the higher energy cost to synthesize their larger glycogen pool compared to PAOI. For both PAOI and PAOII, aerobic conversion rates were more sensitive to salt than anaerobic conversion rates. Potassium (K) and sodium (Na) ions exhibited different effect regardless of the enriched PAO culture, suggesting that the composition of salt is an important factor to consider when studying the effect of salt on EBPR performance.Citation
Wang Z, Dunne A, van Loosdrecht MCM, Saikaly PE (2018) Effect of Salt on the Metabolism of “Candidatus Accumulibacter” Clade I and II. Frontiers in Microbiology 9. Available: http://dx.doi.org/10.3389/fmicb.2018.00479.Sponsors
The authors would like to thank Dr. Laurens Welles at UNESCO-IHE Institute for Water Education for providing PAOI culture.This work was supported by King Abdullah University of Science and Technology (KAUST).Publisher
Frontiers Media SAJournal
Frontiers in MicrobiologyPubMed ID
29616002Additional Links
https://www.frontiersin.org/articles/10.3389/fmicb.2018.00479/fullae974a485f413a2113503eed53cd6c53
10.3389/fmicb.2018.00479
Scopus Count
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