Effect of Salt on the Metabolism of ‘Candidatus Accumulibacter’ Clade I and II
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Environmental Science and Engineering Program
Marine Science Program
Water Desalination and Reuse Research Center (WDRC)
Permanent link to this recordhttp://hdl.handle.net/10754/627386
MetadataShow full item record
AbstractSaline wastewater is known to affect the performance of phosphate-accumulating organisms (PAOs) in enhanced biological phosphorus removal (EBPR) process. However, studies comparing the effect of salinity on different PAO clades are lacking. In this study, 'Candidatus Accumulibacter phosphatis' Clade I and II (hereafter referred to as PAOI and PAOII) were highly enriched (~90% in relative abundance as determined by quantitative FISH) in the form of granules in two sequencing batch reactors. Anaerobic and aerobic batch experiments were conducted to evaluate the effect of salinity on the kinetics and stoichiometry of PAOI and PAOII. PAOI and PAOII communities showed different priority in using polyphosphate (poly-P) and glycogen to generate ATP in the anaerobic phase when exposed to salt, with PAOI depending more on intracellular poly-P degradation (e.g., the proportion of calculated ATP derived from poly-P increased by 5-6% at 0.256 mol/L NaCl or KCl) while PAOII on glycolysis of intracellularly stored glycogen (e.g., the proportion of calculated ATP derived from glycogen increased by 29-30% at 0.256 mol/L NaCl or KCl). In the aerobic phase, the loss of phosphate uptake capability was more pronounced in PAOII due to the higher energy cost to synthesize their larger glycogen pool compared to PAOI. For both PAOI and PAOII, aerobic conversion rates were more sensitive to salt than anaerobic conversion rates. Potassium (K) and sodium (Na) ions exhibited different effect regardless of the enriched PAO culture, suggesting that the composition of salt is an important factor to consider when studying the effect of salt on EBPR performance.
CitationWang Z, Dunne A, van Loosdrecht MCM, Saikaly PE (2018) Effect of Salt on the Metabolism of “Candidatus Accumulibacter” Clade I and II. Frontiers in Microbiology 9. Available: http://dx.doi.org/10.3389/fmicb.2018.00479.
SponsorsThe authors would like to thank Dr. Laurens Welles at UNESCO-IHE Institute for Water Education for providing PAOI culture.This work was supported by King Abdullah University of Science and Technology (KAUST).
PublisherFrontiers Media SA
JournalFrontiers in Microbiology
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Prevalence of 'Candidatus Accumulibacter phosphatis' type II under phosphate limiting conditions.
- Authors: Welles L, Lopez-Vazquez CM, Hooijmans CM, van Loosdrecht MCM, Brdjanovic D
- Issue date: 2016 Dec
- Accumulibacter clades Type I and II performing kinetically different glycogen-accumulating organisms metabolisms for anaerobic substrate uptake.
- Authors: Welles L, Tian WD, Saad S, Abbas B, Lopez-Vazquez CM, Hooijmans CM, van Loosdrecht MC, Brdjanovic D
- Issue date: 2015 Oct 15
- Sulphide effects on the physiology of Candidatus Accumulibacter phosphatis type I.
- Authors: Rubio-Rincón FJ, Lopez-Vazquez CM, Welles L, van Loosdrecht MC, Brdjanovic D
- Issue date: 2017 Feb
- "Candidatus Accumulibacter delftensis": A clade IC novel polyphosphate-accumulating organism without denitrifying activity on nitrate.
- Authors: Rubio-Rincón FJ, Weissbrodt DG, Lopez-Vazquez CM, Welles L, Abbas B, Albertsen M, Nielsen PH, van Loosdrecht MCM, Brdjanovic D
- Issue date: 2019 Sep 15
- Denitrification of nitrate and nitrite by 'Candidatus Accumulibacter phosphatis' clade IC.
- Authors: Saad SA, Welles L, Abbas B, Lopez-Vazquez CM, van Loosdrecht MCM, Brdjanovic D
- Issue date: 2016 Nov 15