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dc.contributor.authorRadecker, Nils
dc.contributor.authorRaina, Jean-Baptiste
dc.contributor.authorPernice, Mathieu
dc.contributor.authorPerna, Gabriela
dc.contributor.authorGuagliardo, Paul
dc.contributor.authorKilburn, Matt R.
dc.contributor.authorAranda, Manuel
dc.contributor.authorVoolstra, Christian R.
dc.date.accessioned2018-03-20T12:34:07Z
dc.date.available2018-03-20T12:34:07Z
dc.date.issued2018-03-16
dc.identifier.citationRädecker N, Raina J-B, Pernice M, Perna G, Guagliardo P, et al. (2018) Using Aiptasia as a Model to Study Metabolic Interactions in Cnidarian-Symbiodinium Symbioses. Frontiers in Physiology 9. Available: http://dx.doi.org/10.3389/fphys.2018.00214.
dc.identifier.issn1664-042X
dc.identifier.pmid29615919
dc.identifier.doi10.3389/fphys.2018.00214
dc.identifier.doi10.3389/fphys.2018.00449
dc.identifier.urihttp://hdl.handle.net/10754/627369
dc.description.abstractThe symbiosis between cnidarian hosts and microalgae of the genus Symbiodinium provides the foundation of coral reefs in oligotrophic waters. Understanding the nutrient-exchange between these partners is key to identifying the fundamental mechanisms behind this symbiosis, yet has proven difficult given the endosymbiotic nature of this relationship. In this study, we investigated the respective contribution of host and symbiont to carbon and nitrogen assimilation in the coral model anemone Aiptaisa. For this, we combined traditional measurements with nanoscale secondary ion mass spectrometry (NanoSIMS) and stable isotope labeling to investigate patterns of nutrient uptake and translocation both at the organismal scale and at the cellular scale. Our results show that the rate of carbon and nitrogen assimilation in Aiptasia depends on the identity of the host and the symbiont. NanoSIMS analysis confirmed that both host and symbiont incorporated carbon and nitrogen into their cells, implying a rapid uptake and cycling of nutrients in this symbiotic relationship. Gross carbon fixation was highest in Aiptasia associated with their native Symbiodinium communities. However, differences in fixation rates were only reflected in the δ13C enrichment of the cnidarian host, whereas the algal symbiont showed stable enrichment levels regardless of host identity. Thereby, our results point toward a “selfish” character of the cnidarian—Symbiodinium association in which both partners directly compete for available resources. Consequently, this symbiosis may be inherently instable and highly susceptible to environmental change. While questions remain regarding the underlying cellular controls of nutrient exchange and the nature of metabolites involved, the approach outlined in this study constitutes a powerful toolset to address these questions.
dc.description.sponsorshipThe authors would like to thank Dr. Rachid Sougrat and Ptissam Bergam from the KAUST imaging core lab for their help with sample preparation. CV and NR acknowledge funding from the KAUST CPF funding. Further, research in this publication was supported by KAUST baseline research funds to CV. The authors would like to acknowledge the Australian Microscopy & Microanalysis Research Facility, AuScope, the Science and Industry Endowment Fund, and the State Government of Western Australian for contributing to the Ion Probe Facility at the Centre for Microscopy, Characterisation and Analysis at the University of Western Australia. J-BR was supported by Australian Research Council fellowship DE160100636.
dc.publisherFrontiers Media SA
dc.relation.urlhttps://www.frontiersin.org/articles/10.3389/fphys.2018.00214/full#h6
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleUsing Aiptasia as a Model to Study Metabolic Interactions in Cnidarian-Symbiodinium Symbioses
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentMarine Science Program
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.identifier.journalFrontiers in Physiology
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionClimate Change Cluster, University of Technology Sydney, Sydney, NSW, Australia
dc.contributor.institutionCentre for Microscopy, Characterisation and Analysis, University of Western Australia, Perth, WA, Australia
kaust.personRadecker, Nils
kaust.personPerna, Gabriela
kaust.personAranda, Manuel
kaust.personVoolstra, Christian R.
refterms.dateFOA2018-06-14T05:08:39Z
kaust.acknowledged.supportUnitKAUST baseline research
kaust.acknowledged.supportUnitKAUST imaging core lab


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This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.