Serano, Natalia Lorena Gorron
Gehring, Christoph A
Reddy, A S N
Mahfouz, Magdy M.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)
Desert Agriculture Initiative
Laboratory for Genome Engineering
Online Publication Date2018-02-27
Print Publication Date2018-04-27
Permanent link to this recordhttp://hdl.handle.net/10754/627209
MetadataShow full item record
AbstractAbiotic and biotic stresses limit crop productivity. Exposure to a non-lethal stress, referred to as priming, can allow plants to survive subsequent and otherwise lethal conditions; the priming effect persists even after a prolonged stress-free period. However, the molecular mechanisms underlying priming are not fully understood. Here, we investigated the molecular basis of heat shock memory and the role of priming in Arabidopsisthaliana. Comprehensive analysis of transcriptome-wide changes in gene expression and alternative splicing in primed and non-primed plants revealed that alternative splicing functions as a novel component of heat shock memory. We show that priming of plants with a non-lethal heat stress results in de-repression of splicing after a second exposure to heat stress. By contrast, non-primed plants showed significant repression of splicing. These observations link ‘splicing memory’ to the ability of plants to survive subsequent and otherwise lethal heat stress. This newly discovered priming-induced splicing memory may represent a general feature of heat stress responses in plants and other organisms as many of the key components of heat shock responses are conserved among eukaryotes. Furthermore, this finding could facilitate the development of novel approaches to improve plant survival under extreme heat stress.
CitationLing Y, Serrano N, Gao G, Atia M, Mokhtar M, et al. (2018) Thermopriming Triggers Splicing Memory in Arabidopsis. Journal of Experimental Botany. Available: http://dx.doi.org/10.1093/jxb/ery062.
SponsorsWe would like to thank members of the Bioscience core facility of KAUST for their help with RNA-sequencing. We would like to thank members of the laboratory for genome engineering for their discussion and technical support. This study was supported by King Abdullah University of Science and Technology (KAUST).
PublisherOxford University Press (OUP)
JournalJournal of Experimental Botany
Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
- BRUSHY1/TONSOKU/MGOUN3 is required for heat stress memory.
- Authors: Brzezinka K, Altmann S, Bäurle I
- Issue date: 2019 Mar
- Distinct heat shock factors and chromatin modifications mediate the organ-autonomous transcriptional memory of heat stress.
- Authors: Liu HC, Lämke J, Lin SY, Hung MJ, Liu KM, Charng YY, Bäurle I
- Issue date: 2018 Aug
- Thermopriming reprograms metabolic homeostasis to confer heat tolerance.
- Authors: Serrano N, Ling Y, Bahieldin A, Mahfouz MM
- Issue date: 2019 Jan 17
- A regulatory role of autophagy for resetting the memory of heat stress in plants.
- Authors: Sedaghatmehr M, Thirumalaikumar VP, Kamranfar I, Marmagne A, Masclaux-Daubresse C, Balazadeh S
- Issue date: 2019 Mar
- Cold priming uncouples light- and cold-regulation of gene expression in Arabidopsis thaliana.
- Authors: Bittner A, van Buer J, Baier M
- Issue date: 2020 Jun 18