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Engineering plant architecture via CRISPR/Cas9-mediated alteration of strigolactone biosynthesis

dc.contributor.authorButt, Haroon
dc.contributor.authorJamil, Muhammad
dc.contributor.authorWang, Jian You
dc.contributor.authorAl-Babili, Salim
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2018-11-25T07:41:04Z
dc.date.available2018-02-07T07:02:28Z
dc.date.available2018-11-25T07:41:04Z
dc.date.issued2018-08-29
dc.identifier.citationButt H, Jamil M, Wang JY, Al-Babili S, Mahfouz M (2018) Engineering plant architecture via CRISPR/Cas9-mediated alteration of strigolactone biosynthesis. BMC Plant Biology 18. Available: http://dx.doi.org/10.1186/s12870-018-1387-1.
dc.identifier.issn1471-2229
dc.identifier.doi10.1186/s12870-018-1387-1
dc.identifier.urihttp://hdl.handle.net/10754/627057
dc.description.abstractPrecision plant genome engineering holds much promise for targeted improvement of crop traits via unprecedented single-base level control over the genetic material. Strigolactones (SLs) are a key determinant of plant architecture, known for their role in inhibiting shoot branching (tillering).We used CRISPR/Cas9 in rice (Oryza sativa) for targeted disruption of CAROTENOID CLEAVAGE DIOXYGENASE 7 (CCD7), which controls a key step in SL biosynthesis. The ccd7 mutants exhibited a striking increase in tillering, combined with a reduced height, which could be rescued by application of the synthetic SL analog GR24. Striga germination assays and liquid chromatography-mass spectrometry analysis showed that root exudates of ccd7 mutants were also SL deficient.Taken together, our results show the potential and feasibility of the use of the CRISPR/Cas9 system for targeted engineering of plant architecture and for elucidating the molecular underpinnings of architecture-related traits.
dc.description.sponsorshipAcknowledgements: We would like to thank members of the laboratory for genome engineering at KAUST for their helpful discussions and critical reading of the manuscript. Funding: This study was supported by King Abdullah University of Science and Technology.
dc.publisherSpringer Nature
dc.relation.urlhttps://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-018-1387-1
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCarotenoids
dc.subjectplant architecture
dc.subjectCrop Improvement
dc.subjectRice Engineering
dc.subjectGenome Editing
dc.subjectStrigolactones
dc.subjectCarotenoid Cleavage Dioxygenases
dc.subjectCrispr/cas9
dc.subjectCcd7
dc.titleEngineering plant architecture via CRISPR/Cas9-mediated alteration of strigolactone biosynthesis
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentLaboratory for Genome Engineering
dc.contributor.departmentPlant Science
dc.identifier.journalBMC Plant Biology
dc.eprint.versionPublisher's Version/PDF
kaust.personButt, Haroon
kaust.personJamil, Muhammad
kaust.personWang, Jian You
kaust.personAl-Babili, Salim
kaust.personMahfouz, Magdy M.
refterms.dateFOA2018-06-13T16:54:03Z
display.relations<b> Is Supplemented By:</b> <br/> <ul> <li><i>[Dataset]</i> <br/> . DOI: <a href="https://doi.org/10.6084/m9.figshare.7027184.v1">10.6084/m9.figshare.7027184.v1</a> HANDLE: <a href="http://hdl.handle.net/10754/664226">10754/664226</a></li></ul><b> Is Supplemented By:</b> <br/> <ul> <li><i>[Dataset]</i> <br/> . DOI: <a href="https://doi.org/10.6084/m9.figshare.c.4215578">10.6084/m9.figshare.c.4215578</a> HANDLE: <a href="http://hdl.handle.net/10754/664227">10754/664227</a></li></ul>
dc.date.published-online2018-08-29
dc.date.published-print2018-12


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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Except where otherwise noted, this item's license is described as This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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VersionItemEditorDateSummary
210754/627057*Han Wang2018-11-25T07:34:26ZPublished with DOI
110754/627057.1Daryl Grenz2018-02-07T07:02:28Z

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