Engineering plant architecture via CRISPR/Cas9-mediated alteration of strigolactone biosynthesis
| dc.contributor.author | Butt, Haroon | |
| dc.contributor.author | Jamil, Muhammad | |
| dc.contributor.author | Wang, Jian You | |
| dc.contributor.author | Al-Babili, Salim | |
| dc.contributor.author | Mahfouz, Magdy M. | |
| dc.date.accessioned | 2018-11-25T07:41:04Z | |
| dc.date.available | 2018-02-07T07:02:28Z | |
| dc.date.available | 2018-11-25T07:41:04Z | |
| dc.date.issued | 2018-08-29 | |
| dc.identifier.citation | Butt H, Jamil M, Wang JY, Al-Babili S, Mahfouz M (2018) Engineering plant architecture via CRISPR/Cas9-mediated alteration of strigolactone biosynthesis. BMC Plant Biology 18. Available: http://dx.doi.org/10.1186/s12870-018-1387-1. | |
| dc.identifier.issn | 1471-2229 | |
| dc.identifier.doi | 10.1186/s12870-018-1387-1 | |
| dc.identifier.doi | 10.1101/254698 | |
| dc.identifier.uri | http://hdl.handle.net/10754/627057 | |
| dc.description.abstract | Precision plant genome engineering holds much promise for targeted improvement of crop traits via unprecedented single-base level control over the genetic material. Strigolactones (SLs) are a key determinant of plant architecture, known for their role in inhibiting shoot branching (tillering).We used CRISPR/Cas9 in rice (Oryza sativa) for targeted disruption of CAROTENOID CLEAVAGE DIOXYGENASE 7 (CCD7), which controls a key step in SL biosynthesis. The ccd7 mutants exhibited a striking increase in tillering, combined with a reduced height, which could be rescued by application of the synthetic SL analog GR24. Striga germination assays and liquid chromatography-mass spectrometry analysis showed that root exudates of ccd7 mutants were also SL deficient.Taken together, our results show the potential and feasibility of the use of the CRISPR/Cas9 system for targeted engineering of plant architecture and for elucidating the molecular underpinnings of architecture-related traits. | |
| dc.description.sponsorship | Acknowledgements: We would like to thank members of the laboratory for genome engineering at KAUST for their helpful discussions and critical reading of the manuscript. Funding: This study was supported by King Abdullah University of Science and Technology. | |
| dc.publisher | Springer Nature | |
| dc.relation.url | https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-018-1387-1 | |
| dc.rights | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Carotenoids | |
| dc.subject | plant architecture | |
| dc.subject | Crop Improvement | |
| dc.subject | Rice Engineering | |
| dc.subject | Genome Editing | |
| dc.subject | Strigolactones | |
| dc.subject | Carotenoid Cleavage Dioxygenases | |
| dc.subject | Crispr/cas9 | |
| dc.subject | Ccd7 | |
| dc.title | Engineering plant architecture via CRISPR/Cas9-mediated alteration of strigolactone biosynthesis | |
| dc.type | Article | |
| dc.contributor.department | Biological and Environmental Science and Engineering (BESE) Division | |
| dc.contributor.department | Bioscience Program | |
| dc.contributor.department | Center for Desert Agriculture | |
| dc.contributor.department | Laboratory for Genome Engineering | |
| dc.contributor.department | Plant Science | |
| dc.identifier.journal | BMC Plant Biology | |
| dc.eprint.version | Publisher's Version/PDF | |
| kaust.person | Butt, Haroon | |
| kaust.person | Jamil, Muhammad | |
| kaust.person | Wang, Jian You | |
| kaust.person | Al-Babili, Salim | |
| kaust.person | Mahfouz, Magdy M. | |
| dc.relation.issupplementedby | DOI:10.6084/m9.figshare.7027184.v1 | |
| dc.relation.issupplementedby | DOI:10.6084/m9.figshare.c.4215578 | |
| refterms.dateFOA | 2018-06-13T16:54:03Z | |
| display.relations | <b> Is Supplemented By:</b> <br/> <ul> <li><i>[Dataset]</i> <br/> . DOI: <a href="https://doi.org/10.6084/m9.figshare.7027184.v1">10.6084/m9.figshare.7027184.v1</a> HANDLE: <a href="http://hdl.handle.net/10754/664226">10754/664226</a></li></ul><b> Is Supplemented By:</b> <br/> <ul> <li><i>[Dataset]</i> <br/> . DOI: <a href="https://doi.org/10.6084/m9.figshare.c.4215578">10.6084/m9.figshare.c.4215578</a> HANDLE: <a href="http://hdl.handle.net/10754/664227">10754/664227</a></li></ul> | |
| dc.date.published-online | 2018-08-29 | |
| dc.date.published-print | 2018-12 |
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