Engineering Plant Architecture via CRISPR/Cas9-mediated Alteration of Strigolactone Biosynthesis
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Plant Science Program
Desert Agriculture Initiative
Laboratory for Genome Engineering
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AbstractPrecision plant genome engineering holds much promise for targeted improvement of crop traits via unprecedented single-base level control over the genetic material. Strigolactones (SLs) are a key determinant of plant architecture, known for their role in inhibiting shoot branching (tillering). Here, we used CRISPR/Cas9 in rice (Oryza sativa) for targeted disruption of CAROTENOID CLEAVAGE DIOXYGENASE 7 (CCD7), which controls a key step in SL biosynthesis. The ccd7 mutants exhibited a striking increase in tillering, combined with a dwarf phenotype, which could be rescued by application of the synthetic SL analog GR24. Striga germination assays and liquid chromatography mass spectrometry analysis showed that root exudates of ccd7 mutants were also SL deficient. Taken together, our results show the power of CRISPR/Cas9 for targeted engineering of plant architecture and for elucidating the molecular underpinnings of architecture-related traits.
CitationButt H, Jamil M, Wang JY, Al-Babili S, Mahfouz M (2018) Engineering Plant Architecture via CRISPR/Cas9-mediated Alteration of Strigolactone Biosynthesis. Available: http://dx.doi.org/10.1101/254698.
SponsorsWe would like to thank members of the laboratory for genome engineering at KAUST for their helpful discussions and critical reading of the manuscript. This study was supported by King Abdullah University of Science and Technology.
PublisherCold Spring Harbor Laboratory