Intramolecular Crosstalk between Catalytic Activities of Receptor Kinases
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Online Publication Date2018-02-15
Print Publication Date2018-02
Permanent link to this recordhttp://hdl.handle.net/10754/627011
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AbstractSignal modulation is important for the growth and development of plants and this process is mediated by a number of factors including physiological growth regulators and their associated signal transduction pathways. Protein kinases play a central role in signaling, including those involving pathogen response mechanisms. We previously demonstrated an active guanylate cyclase (GC) catalytic center in the brassinosteroid insensitive receptor (AtBRI1) within an active intracellular kinase domain resulting in dual enzymatic activity. Here we propose a novel type of receptor architecture that is characterized by a functional GC catalytic center nested in the cytosolic kinase domain enabling intramolecular crosstalk. This may be through a cGMP-AtBRI1 complex forming that may induce a negative feedback mechanism leading to desensitisation of the receptor, regulated through the cGMP production pathway. We further argue that the comparatively low but highly localized cGMP generated by the GC in response to a ligand is sufficient to modulate the kinase activity. This type of receptor therefore provides a molecular switch that directly and/or indirectly affects ligand dependent phosphorylation of downstream signaling cascades and suggests that subsequent signal transduction and modulation works in conjunction with the kinase in downstream signaling.
CitationKwezi L, Wheeler JI, Marondedze C, Gehring C, Irving HR (2018) Intramolecular Crosstalk between Catalytic Activities of Receptor Kinases. Plant Signaling & Behavior: 00–00. Available: http://dx.doi.org/10.1080/15592324.2018.1430544.
SponsorsFunding for this research was provided by the Australian Research Council's Discovery funding scheme (project numbers DP0878194 and DP110104164) and the National Research Foundation South Africa (grant numbers 78843; IRF2009021800047).
PublisherInforma UK Limited
JournalPlant Signaling & Behavior
- The brassinosteroid receptor BRI1 can generate cGMP enabling cGMP-dependent downstream signaling.
- Authors: Wheeler JI, Wong A, Marondedze C, Groen AJ, Kwezi L, Freihat L, Vyas J, Raji MA, Irving HR, Gehring C
- Issue date: 2017 Aug
- The phytosulfokine (PSK) receptor is capable of guanylate cyclase activity and enabling cyclic GMP-dependent signaling in plants.
- Authors: Kwezi L, Ruzvidzo O, Wheeler JI, Govender K, Iacuone S, Thompson PE, Gehring C, Irving HR
- Issue date: 2011 Jun 24
- Calcium is the switch in the moonlighting dual function of the ligand-activated receptor kinase phytosulfokine receptor 1.
- Authors: Muleya V, Wheeler JI, Ruzvidzo O, Freihat L, Manallack DT, Gehring C, Irving HR
- Issue date: 2014 Sep 23
- Moonlighting kinases with guanylate cyclase activity can tune regulatory signal networks.
- Authors: Irving HR, Kwezi L, Wheeler J, Gehring C
- Issue date: 2012 Feb
- Phosphorylation of the dimeric cytoplasmic domain of the phytosulfokine receptor, PSKR1.
- Authors: Muleya V, Marondedze C, Wheeler JI, Thomas L, Mok YF, Griffin MD, Manallack DT, Kwezi L, Lilley KS, Gehring C, Irving HR
- Issue date: 2016 Oct 1
Showing items related by title, author, creator and subject.
Calcium is the switch in the moonlighting dual function of the ligand-activated receptor kinase phytosulfokine receptor 1Muleya, Victor; Wheeler, Janet I; Ruzvidzo, Oziniel; Freihat, Lubna; Manallack, David T; Gehring, Christoph A; Irving, Helen R (Cell Communication and Signaling, Springer Nature, 2014-09-23) [Article]Background: A number of receptor kinases contain guanylate cyclase (GC) catalytic centres encapsulated in the cytosolic kinase domain. A prototypical example is the phytosulfokine receptor 1 (PSKR1) that is involved in regulating growth responses in plants. PSKR1 contains both kinase and GC activities however the underlying mechanisms regulating the dual functions have remained elusive. Findings: Here, we confirm the dual activity of the cytoplasmic domain of the PSKR1 receptor. We show that mutations within the guanylate cyclase centre modulate the GC activity while not affecting the kinase catalytic activity. Using physiologically relevant Ca2+ levels, we demonstrate that its GC activity is enhanced over two-fold by Ca2+ in a concentration-dependent manner. Conversely, increasing Ca2+ levels inhibits kinase activity up to 500-fold at 100 nM Ca2+. Conclusions: Changes in calcium at physiological levels can regulate the kinase and GC activities of PSKR1. We therefore propose a functional model of how calcium acts as a bimodal switch between kinase and GC activity in PSKR1 that could be relevant to other members of this novel class of ligand-activated receptor kinases.
Phosphorylation of the dimeric cytoplasmic domain of the phytosulfokine receptor, PSKR1Muleya, V.; Marondedze, Claudius; Wheeler, J. I.; Thomas, Ludivine; Mok, Y.-F.; Griffin, M. D. W.; Manallack, D. T.; Kwezi, L.; Lilley, K. S.; Gehring, Christoph A; Irving, H. R. (Biochemical Journal, Portland Press Ltd., 2016-08-03) [Article]Phytosulfokines (PSKs) are plant peptide hormones that co-regulate plant growth, differentiation and defense responses. PSKs signal through a plasma membrane localized leucine-rich repeat receptor-like kinase (phytosulfokine receptor 1, PSKR1) that also contains a functional cytosolic guanylate cyclase with its cyclase catalytic center embedded within the kinase domain. To functionally characterize this novel type of overlapping dual catalytic function, we investigated the phosphorylation of PSKR1 in vitro Tandem mass spectrometry of the cytoplasmic domain of PSKR1 (PSKR1cd) revealed at least 11 phosphorylation sites (8 serines, 2 threonines and 1 tyrosine) within the PSKR1cd. Phosphomimetic mutations of three serine residues (Ser686, Ser696 and Ser698) in tandem at the juxta-membrane position resulted in enhanced kinase activity in the on-mutant that was suppressed in the off-mutant, but both mutations reduced guanylate cyclase activity. Both the on and off phosphomimetic mutations of the phosphotyrosine (Tyr888) residue in the activation loop suppressed kinase activity, while neither mutation affected guanylate cyclase activity. Size exclusion and analytical ultracentrifugation analysis of the PSKR1cd suggest that it is reversibly dimeric in solution, which was further confirmed by biflourescence complementation. Taken together, these data suggest that in this novel type of receptor domain architecture, specific phosphorylation and dimerization are possibly essential mechanisms for ligand-mediated catalysis and signaling.
Dual action of L-Lactate on the activity of NR2B-containing NMDA receptors: from potentiation to neuroprotectionJourdain, P.; Rothenfusser, K.; Ben-Adiba, C.; Allaman, I.; Marquet, P.; Magistretti, Pierre J. (Scientific Reports, Springer Nature, 2018-09-07) [Article]L-Lactate is a positive modulator of NMDAR-mediated signaling resulting in plasticity gene induction and memory consolidation. However, L-Lactate is also able to protect neurons against excito-toxic NMDAR activity, an indication of a mitigating action of L-Lactate on NMDA signaling. In this study, we provide experimental evidence that resolves this apparent paradox. Transient co-application of glutamate/glycine (1 μM/100 μM; 2 min) in primary cultures of mouse cortical neurons triggers a NMDA-dependent Ca2+ signal positively modulated by L-Lactate (10 mM) or DTT (1 mM) but decreased by Pyruvate (10 mM). This L-Lactate and DTT-induced potentiation is blocked by Ifenprodil (2 μM), a specific blocker of NMDARs containing NR2B sub-units. In contrast, co-application of glutamate/glycine (1 mM/100 μM; 2 min) elicits a NMDAR-dependent excitotoxic death in 49% of neurons. L-Lactate and Pyruvate significantly reduce this rate of cell death processes (respectively to 23% and 9%) while DTT has no effect (54% of neuronal death). This L-Lactate-induced neuroprotection is blocked by carbenoxolone and glibenclamide, respectively blockers of pannexins and KATP. In conclusion, our results show that L-Lactate is involved in two distinct and independent pathways defined as NMDAR-mediated potentiation pathway (or NADH pathway) and a neuroprotective pathway (or Pyruvate/ATP pathway), the prevalence of each one depending on the strength of the glutamatergic stimulus.