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dc.contributor.authorMarasco, Ramona
dc.contributor.authorRolli, Eleonora
dc.contributor.authorFusi, Marco
dc.contributor.authorMichoud, Gregoire
dc.contributor.authorDaffonchio, Daniele
dc.date.accessioned2018-01-28T07:01:36Z
dc.date.available2018-01-28T07:01:36Z
dc.date.issued2018-01-03
dc.identifier.citationMarasco R, Rolli E, Fusi M, Michoud G, Daffonchio D (2018) Grapevine rootstocks shape underground bacterial microbiome and networking but not potential functionality. Microbiome 6. Available: http://dx.doi.org/10.1186/s40168-017-0391-2.
dc.identifier.issn2049-2618
dc.identifier.pmid29298729
dc.identifier.doi10.1186/s40168-017-0391-2
dc.identifier.urihttp://hdl.handle.net/10754/626863
dc.description.abstractThe plant compartments of Vitis vinifera, including the rhizosphere, rhizoplane, root endosphere, phyllosphere and carposphere, provide unique niches that drive specific bacterial microbiome associations. The majority of phyllosphere endophytes originate from the soil and migrate up to the aerial compartments through the root endosphere. Thus, the soil and root endosphere partially define the aerial endosphere in the leaves and berries, contributing to the terroir of the fruit. However, V. vinifera cultivars are invariably grafted onto the rootstocks of other Vitis species and hybrids. It has been hypothesized that the plant species determines the microbiome of the root endosphere and, as a consequence, the aerial endosphere. In this work, we test the first part of this hypothesis. We investigate whether different rootstocks influence the bacteria selected from the surrounding soil, affecting the bacterial diversity and potential functionality of the rhizosphere and root endosphere.Bacterial microbiomes from both the root tissues and the rhizosphere of Barbera cultivars, both ungrafted and grafted on four different rootstocks, cultivated in the same soil from the same vineyard, were characterized by 16S rRNA high-throughput sequencing. To assess the influence of the root genotype on the bacterial communities' recruitment in the root system, (i) the phylogenetic diversity coupled with the predicted functional profiles and (ii) the co-occurrence bacterial networks were determined. Cultivation-dependent approaches were used to reveal the plant-growth promoting (PGP) potential associated with the grafted and ungrafted root systems.Richness, diversity and bacterial community networking in the root compartments were significantly influenced by the rootstocks. Complementary to a shared bacterial microbiome, different subsets of soil bacteria, including those endowed with PGP traits, were selected by the root system compartments of different rootstocks. The interaction between the root compartments and the rootstock exerted a unique selective pressure that enhanced niche differentiation, but rootstock-specific bacterial communities were still recruited with conserved PGP traits.While the rootstock significantly influences the taxonomy, structure and network properties of the bacterial community in grapevine roots, a homeostatic effect on the distribution of the predicted and potential functional PGP traits was found.
dc.description.sponsorshipAcknowledgements: The authors thank Roberto Gerbino (Le Fracce Farm, Fondazione Bussolera Branca, Casteggio, Italy) for the support in grapevine sampling. The authors are particularly grateful to Karoline Faust for essential help in network analysis and to Dr. Luigimaria Borruso for his useful advice. Funding: This research was financially supported by the University of Milan and by the King Abdullah University of Science and Technology (KAUST). ER acknowledges support by Università degli Studi di Milano, DeFENS, the European Social Fund (FSE) and Regione Lombardia (contract ‘Dote Ricerca’).
dc.publisherSpringer Nature
dc.relation.urlhttps://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-017-0391-2
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectRhizosphere
dc.subjectGrapevine
dc.subjectMicrobial ecology
dc.subjectMicrobiome
dc.subjectCo-occurrence Network
dc.subjectEndosphere
dc.subjectPlant Growth-promoting Bacteria
dc.subjectBacterial Recruitment
dc.subjectRootstock Selection
dc.titleGrapevine rootstocks shape underground bacterial microbiome and networking but not potential functionality
dc.typeArticle
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.identifier.journalMicrobiome
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Food Environmental and Nutritional Sciences, Università degli Studi di Milano, 20133, Milano, Italy.
kaust.personMarasco, Ramona
kaust.personFusi, Marco
kaust.personMichoud, Gregoire
kaust.personDaffonchio, Daniele
refterms.dateFOA2018-06-14T03:42:56Z
display.relations<b> Is Supplemented By:</b> <br/> <ul> <li><i>[Dataset]</i> <br/> . DOI: <a href="https://doi.org/10.6084/m9.figshare.5755572.v1">10.6084/m9.figshare.5755572.v1</a> HANDLE: <a href="http://hdl.handle.net/10754/663981">10754/663981</a></li></ul><b> Is Supplemented By:</b> <br/> <ul> <li><i>[Dataset]</i> <br/> . DOI: <a href="https://doi.org/10.6084/m9.figshare.c.3967767">10.6084/m9.figshare.c.3967767</a> HANDLE: <a href="http://hdl.handle.net/10754/663982">10754/663982</a></li></ul>
dc.date.published-online2018-01-03
dc.date.published-print2018-12


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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Except where otherwise noted, this item's license is described as This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.