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dc.contributor.authorDöll, Stefanie
dc.contributor.authorKuhlmann, Markus
dc.contributor.authorRutten, Twan
dc.contributor.authorMette, Michael F.
dc.contributor.authorScharfenberg, Sarah
dc.contributor.authorPetridis, Antonios
dc.contributor.authorBerreth, Dorothee-Carina
dc.contributor.authorMock, Hans-Peter
dc.date.accessioned2018-01-18T08:32:08Z
dc.date.available2018-01-18T08:32:08Z
dc.date.issued2018-01-10
dc.identifier.citationDöll S, Kuhlmann M, Rutten T, Mette MF, Scharfenberg S, et al. (2018) Accumulation of the coumarin scopolin under abiotic stress conditions is mediated by the Arabidopsis thaliana THO/TREX complex. The Plant Journal. Available: http://dx.doi.org/10.1111/tpj.13797.
dc.identifier.issn0960-7412
dc.identifier.doi10.1111/tpj.13797
dc.identifier.urihttp://hdl.handle.net/10754/626837
dc.description.abstractSecondary metabolites are involved in the plant stress response. Among these are scopolin and its active form scopoletin, which are coumarin derivatives associated with reactive oxygen species scavenging and pathogen defence. Here we show that scopolin accumulation can be induced in the root by osmotic stress and in the leaf by low-temperature stress in Arabidopsis thaliana. A genetic screen for altered scopolin levels in A. thaliana revealed a mutant compromised in scopolin accumulation in response to stress; the lesion was present in a homologue of THO1 coding for a subunit of the THO/TREX complex. The THO/TREX complex contributes to RNA silencing, supposedly by trafficking precursors of small RNAs. Mutants defective in THO, AGO1, SDS3 and RDR6 were impaired with respect to scopolin accumulation in response to stress, suggesting a mechanism based on RNA silencing such as the trans-acting small interfering RNA pathway, which requires THO/TREX function.
dc.description.sponsorshipFunding of research by grants to H-P Mock from the EU (ATHENA; KBBE-2009-13) and the BMBF (FKZ 031A108B) are gratefully acknowledged. Antonios Petridis was supported by a post-doctoral fellowship from the Leibniz Association and the German Academic Exchange Service (DAAD). We thank the Salk Institute Genomic Analysis Laboratory for providing the sequence-indexed Arabidopsis T-DNA insertion mutants. Funding for the SIGnAL indexed insertion mutant collection was provided by the National Science Foundation. We also thank the Nottingham Arabidopsis Stock Centre, the GABI-KAT consortium and INRA Versailles for providing further seed stocks. Furthermore we are grateful for the assistance of Katja Witzel (Leibniz Institute of Vegetable and Ornamental Crops, Großbeeren, Germany) with the transcript analysis. We would also like to thank Steffen Neumann (Leibniz Institute for Plant Biochemistry, Halle, Germany) for the upload of LC-MS data into the MetaboLights database.
dc.publisherWiley
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1111/tpj.13797/full
dc.rightsThis is the peer reviewed version of the following article: Accumulation of the coumarin scopolin under abiotic stress conditions is mediated by the Arabidopsis thaliana THO/TREX complex, which has been published in final form at http://doi.org/10.1111/tpj.13797. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
dc.subjectAbiotic stress
dc.subjectArabidopsis thaliana
dc.subjectCold stress
dc.subjectOsmotic stress
dc.subjectRDR6 (AT3G49500)
dc.subjectRNA sequencing
dc.subjectRNA silencing
dc.subjectScopolin
dc.subjectTHO1/HPR1/EMU (At5g09860)
dc.titleAccumulation of the coumarin scopolin under abiotic stress conditions is mediated by the Arabidopsis thaliana THO/TREX complex
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalThe Plant Journal
dc.eprint.versionPost-print
dc.contributor.institutionStress and Developmental Biology; Leibniz Institute of Plant Biochemistry (IPB); Weinberg 3 06120 Halle (Saale) Germany
dc.contributor.institutionDepartment of Physiology and Cell Biology; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstraße 3 06466 Seeland OT Gatersleben Germany
dc.contributor.institutionDepartment of Molecular Genetics; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstraße 3 06466 Seeland OT Gatersleben Germany
dc.contributor.institutionDepartment of Breeding Research; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstraße 3 06466 Seeland OT Gatersleben Germany
dc.contributor.institutionJames Hutton Limited Dundee, Invergowrie, Dundee, DD2 5DA, UK
kaust.personMette, Michael F.
refterms.dateFOA2019-01-10T00:00:00Z
dc.date.published-online2018-01-10
dc.date.published-print2018-02


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