Development of inhibitory synaptic inputs on layer 2/3 pyramidal neurons in the rat medial prefrontal cortex
dc.contributor.author | Virtanen, Mari A. | |
dc.contributor.author | Lacoh, Claudia Marvine | |
dc.contributor.author | Fiumelli, Hubert | |
dc.contributor.author | Kosel, Markus | |
dc.contributor.author | Tyagarajan, Shiva | |
dc.contributor.author | de Roo, Mathias | |
dc.contributor.author | Vutskits, Laszlo | |
dc.date.accessioned | 2018-01-16T13:28:37Z | |
dc.date.available | 2018-01-16T13:28:37Z | |
dc.date.issued | 2018-01-10 | |
dc.identifier.citation | Virtanen MA, Lacoh CM, Fiumelli H, Kosel M, Tyagarajan S, et al. (2018) Development of inhibitory synaptic inputs on layer 2/3 pyramidal neurons in the rat medial prefrontal cortex. Brain Structure and Function. Available: http://dx.doi.org/10.1007/s00429-017-1602-0. | |
dc.identifier.issn | 1863-2653 | |
dc.identifier.issn | 1863-2661 | |
dc.identifier.pmid | 29322238 | |
dc.identifier.doi | 10.1007/s00429-017-1602-0 | |
dc.identifier.uri | http://hdl.handle.net/10754/626826 | |
dc.description.abstract | Inhibitory control of pyramidal neurons plays a major role in governing the excitability in the brain. While spatial mapping of inhibitory inputs onto pyramidal neurons would provide important structural data on neuronal signaling, studying their distribution at the single cell level is difficult due to the lack of easily identifiable anatomical proxies. Here, we describe an approach where in utero electroporation of a plasmid encoding for fluorescently tagged gephyrin into the precursors of pyramidal cells along with ionotophoretic injection of Lucifer Yellow can reliably and specifically detect GABAergic synapses on the dendritic arbour of single pyramidal neurons. Using this technique and focusing on the basal dendritic arbour of layer 2/3 pyramidal cells of the medial prefrontal cortex, we demonstrate an intense development of GABAergic inputs onto these cells between postnatal days 10 and 20. While the spatial distribution of gephyrin clusters was not affected by the distance from the cell body at postnatal day 10, we found that distal dendritic segments appeared to have a higher gephyrin density at later developmental stages. We also show a transient increase around postnatal day 20 in the percentage of spines that are carrying a gephyrin cluster, indicative of innervation by a GABAergic terminal. Since the precise spatial arrangement of synaptic inputs is an important determinant of neuronal responses, we believe that the method described in this work may allow a better understanding of how inhibition settles together with excitation, and serve as basics for further modelling studies focusing on the geometry of dendritic inhibition during development. | |
dc.description.sponsorship | We thank Michèle Brunet for excellent technical assistance, Kai Kaila, Jean-Marc Fritschy and Jozsef Kiss for instructive comments on the manuscript. This study was funded by the Swiss National Science Foundation, Berne, Switzerland, Grant 31003A-130625 (to LV). | |
dc.publisher | Springer Nature | |
dc.relation.url | http://link.springer.com/article/10.1007/s00429-017-1602-0 | |
dc.rights | The final publication is available at Springer via http://dx.doi.org/10.1007/s00429-017-1602-0 | |
dc.subject | Development | |
dc.subject | Cerebral cortex | |
dc.subject | GABA | |
dc.subject | Synaptogenesis | |
dc.subject | Gephyrin | |
dc.title | Development of inhibitory synaptic inputs on layer 2/3 pyramidal neurons in the rat medial prefrontal cortex | |
dc.type | Article | |
dc.contributor.department | Biological and Environmental Sciences and Engineering (BESE) Division | |
dc.identifier.journal | Brain Structure and Function | |
dc.eprint.version | Post-print | |
dc.contributor.institution | Department of Biosciences, University of Helsinki, Helsinki, Finland | |
dc.contributor.institution | Department of Basic Neuroscience, University of Geneva Medical School, Geneva, Switzerland. | |
dc.contributor.institution | Department of Psychiatry, University of Geneva Medical School, Geneva, Switzerland. | |
dc.contributor.institution | Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland. | |
dc.contributor.institution | Department of Anesthesiology, Pharmacology and Intensive Care, University Hospitals of Geneva, Geneva 4, Switzerland | |
kaust.person | Fiumelli, Hubert | |
refterms.dateFOA | 2019-01-10T00:00:00Z |
Files in this item
This item appears in the following Collection(s)
-
Articles
-
Biological and Environmental Science and Engineering (BESE) Division
For more information visit: https://bese.kaust.edu.sa/