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dc.contributor.authorVirtanen, Mari A.
dc.contributor.authorLacoh, Claudia Marvine
dc.contributor.authorFiumelli, Hubert
dc.contributor.authorKosel, Markus
dc.contributor.authorTyagarajan, Shiva
dc.contributor.authorde Roo, Mathias
dc.contributor.authorVutskits, Laszlo
dc.date.accessioned2018-01-16T13:28:37Z
dc.date.available2018-01-16T13:28:37Z
dc.date.issued2018-01-10
dc.identifier.citationVirtanen MA, Lacoh CM, Fiumelli H, Kosel M, Tyagarajan S, et al. (2018) Development of inhibitory synaptic inputs on layer 2/3 pyramidal neurons in the rat medial prefrontal cortex. Brain Structure and Function. Available: http://dx.doi.org/10.1007/s00429-017-1602-0.
dc.identifier.issn1863-2653
dc.identifier.issn1863-2661
dc.identifier.pmid29322238
dc.identifier.doi10.1007/s00429-017-1602-0
dc.identifier.urihttp://hdl.handle.net/10754/626826
dc.description.abstractInhibitory control of pyramidal neurons plays a major role in governing the excitability in the brain. While spatial mapping of inhibitory inputs onto pyramidal neurons would provide important structural data on neuronal signaling, studying their distribution at the single cell level is difficult due to the lack of easily identifiable anatomical proxies. Here, we describe an approach where in utero electroporation of a plasmid encoding for fluorescently tagged gephyrin into the precursors of pyramidal cells along with ionotophoretic injection of Lucifer Yellow can reliably and specifically detect GABAergic synapses on the dendritic arbour of single pyramidal neurons. Using this technique and focusing on the basal dendritic arbour of layer 2/3 pyramidal cells of the medial prefrontal cortex, we demonstrate an intense development of GABAergic inputs onto these cells between postnatal days 10 and 20. While the spatial distribution of gephyrin clusters was not affected by the distance from the cell body at postnatal day 10, we found that distal dendritic segments appeared to have a higher gephyrin density at later developmental stages. We also show a transient increase around postnatal day 20 in the percentage of spines that are carrying a gephyrin cluster, indicative of innervation by a GABAergic terminal. Since the precise spatial arrangement of synaptic inputs is an important determinant of neuronal responses, we believe that the method described in this work may allow a better understanding of how inhibition settles together with excitation, and serve as basics for further modelling studies focusing on the geometry of dendritic inhibition during development.
dc.description.sponsorshipWe thank Michèle Brunet for excellent technical assistance, Kai Kaila, Jean-Marc Fritschy and Jozsef Kiss for instructive comments on the manuscript. This study was funded by the Swiss National Science Foundation, Berne, Switzerland, Grant 31003A-130625 (to LV).
dc.publisherSpringer Nature
dc.relation.urlhttp://link.springer.com/article/10.1007/s00429-017-1602-0
dc.rightsThe final publication is available at Springer via http://dx.doi.org/10.1007/s00429-017-1602-0
dc.subjectDevelopment
dc.subjectCerebral cortex
dc.subjectGABA
dc.subjectSynaptogenesis
dc.subjectGephyrin
dc.titleDevelopment of inhibitory synaptic inputs on layer 2/3 pyramidal neurons in the rat medial prefrontal cortex
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalBrain Structure and Function
dc.eprint.versionPost-print
dc.contributor.institutionDepartment of Biosciences, University of Helsinki, Helsinki, Finland
dc.contributor.institutionDepartment of Basic Neuroscience, University of Geneva Medical School, Geneva, Switzerland.
dc.contributor.institutionDepartment of Psychiatry, University of Geneva Medical School, Geneva, Switzerland.
dc.contributor.institutionInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.
dc.contributor.institutionDepartment of Anesthesiology, Pharmacology and Intensive Care, University Hospitals of Geneva, Geneva 4, Switzerland
kaust.personFiumelli, Hubert
refterms.dateFOA2019-01-10T00:00:00Z


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