Not just a marker: CD34 on human hematopoietic stem/progenitor cells dominates vascular selectin binding along with CD44
AuthorsAbu Samra, Dina Bashir Kamil
Aleisa, Fajr A
Al-Amoodi, Asma S.
Jalal Ahmed, Heba M.
Chin, Chee Jia
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Physical Sciences and Engineering (PSE) Division
Chemical and Biological Engineering Program
Imaging and Characterization Core Lab
KAUST Grant NumberCRG_R2_13_MERZ_KAUST_1
Permanent link to this recordhttp://hdl.handle.net/10754/626762
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AbstractCD34 is routinely used to identify and isolate human hematopoietic stem/progenitor cells (HSPCs) for use clinically in bone marrow transplantation, but its function on these cells remains elusive. Glycoprotein ligands on HSPCs help guide their migration to specialized microvascular beds in the bone marrow that express vascular selectins (E- and P-selectin). Here, we show that HSPC-enriched fractions from human hematopoietic tissue expressing CD34 (CD34pos) bound selectins, whereas those lacking CD34 (CD34neg) did not. An unbiased proteomics screen identified potential glycoprotein ligands on CD34pos cells revealing CD34 itself as a major vascular selectin ligand. Biochemical and CD34 knockdown analyses highlight a key role for CD34 in the first prerequisite step of cell migration, suggesting that it is not just a marker on these cells. Our results also entice future potential strategies to investigate the glycoforms of CD34 that discriminate normal HSPCs from leukemic cells and to manipulate CD34neg HSPC-enriched bone marrow or cord blood populations as a source of stem cells for clinical use.
CitationAbuSamra DB, Aleisa FA, Al-Amoodi AS, Jalal Ahmed HM, Chin CJ, et al. (2017) Not just a marker: CD34 on human hematopoietic stem/progenitor cells dominates vascular selectin binding along with CD44. Blood Advances 1: 2799–2816. Available: http://dx.doi.org/10.1182/bloodadvances.2017004317.
SponsorsThe authors sincerely thank Samah Z. Gadhoum for her help and support throughout the project and Samir Hamdan for his time and fruitful discussions regarding the SPR analyses. The authors express their gratitude to Maryam Mih and Samar A. Rustum for their support in the management of the laboratory and also to Mohammad Imran Khan for his assistance with the confocal microscopy. Wei Xu at Imaging and Characterization core facility at King Abdullah University of Science and Technology (KAUST) was very helpful at providing analysis software and training on the confocal microscopes. The authors also thank Carolyn Unck from the KAUST Academic Writing Service for editing the manuscript. In addition, a special thanks to Aswini K. Panigrahi (MS assistance) and Alaguraj Dharmarajnadar (cell sorting on BD Influx) from the Bioscience Core Laboratory Facility at KAUST. This research was supported by a KAUST Faculty Baseline Research Funding Program and by a Competitive Research Grant (CRG_R2_13_MERZ_KAUST_1) (J.S.M.).
PublisherAmerican Society of Hematology
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