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dc.contributor.authorShaw, Dario Rangel
dc.contributor.authorDussan, Jenny
dc.date.accessioned2018-01-01T12:19:01Z
dc.date.available2018-01-01T12:19:01Z
dc.date.issued2017-11-23
dc.identifier.citationShaw DR, Dussan J (2018) Transcriptional analysis and molecular dynamics simulations reveal the mechanism of toxic metals removal and efflux pumps in Lysinibacillus sphaericus OT4b.31. International Biodeterioration & Biodegradation 127: 46–61. Available: http://dx.doi.org/10.1016/j.ibiod.2017.11.016.
dc.identifier.issn0964-8305
dc.identifier.doi10.1016/j.ibiod.2017.11.016
dc.identifier.urihttp://hdl.handle.net/10754/626593
dc.description.abstractLysinibacillus sphaericus strain OT4b.31 is a bacterium widely applied in bioremediation processes of hydrocarbon and metal polluted environments. In this study, we identified the molecular mechanism underlying the Pb2+ and Cr6+ resistance. Metal uptake and temporal transcription patterns of metal resistance operons were evaluated using reverse-transcribed quantitative PCR amplification. The function of the resistance determinants was studied applying docking and in silico mutagenesis methods. The results revealed that the adaptation of Lysinibacillus sphaericus OT4b.31 to elevated levels of lead and chromium involves the pbr and chr operons which comprise a transcriptional regulatory component (pbrR and chrB) and efflux ATPases (pbrA and chrA) to expel ions from the cytoplasm. Expression of metal resistance genes was constitutive and specifically inducible to the exposure of Pb2+ and Cr6+. The simultaneous presence of cations didn't affect the bioaccumulation of metals, evidencing the multimetal resistance of L. sphaericus. Docking analysis revealed the key metal-protein interactions and the conformational changes after metal or ATP binding. Results showed that residues with aromatic rings or imidazole in the catalytic domain are crucial for metal binding and achievement of the function. To our knowledge, this is the first report of a specific mechanism for lead and chromium resistance in Lysinibacillus genus. From the findings of this study, it is possible to suggest the bacterium as a suitable candidate for rapid toxic metals bioremediation processes.
dc.description.sponsorshipThis work was done with grant funds provided by the Microbiological Research Center – CIMIC and the Research Committee of the Science Faculty at Universidad de Los Andes, Colombia.
dc.publisherElsevier BV
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0964830517308363
dc.subjectLysinibacillus sphaericus
dc.subjectEfflux pumps
dc.subjectLead
dc.subjectChromium
dc.subjectRT-qPCR
dc.subjectDocking
dc.titleTranscriptional analysis and molecular dynamics simulations reveal the mechanism of toxic metals removal and efflux pumps in Lysinibacillus sphaericus OT4b.31
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)
dc.identifier.journalInternational Biodeterioration & Biodegradation
dc.contributor.institutionCenter of Microbiological Research– CIMIC, University of Los Andes, Bogota, Colombia
kaust.personShaw, Dario Rangel


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