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    Label-free three-dimensional (3D) structural imaging in live cells using intrinsic optical refractive index

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    1704.04445.pdf
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    Description:
    Preprint
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    Type
    Preprint
    Authors
    Liu, Chen
    Malek, Michael
    Poon, Ivan K. H.
    Jiang, Lanzhou
    Sheppard, Colin J. R.
    Roberts, Ann
    Quiney, Harry
    Zhang, Douguo
    Yuan, Xiaocong
    Lin, Jiao
    Depeursinge, Christian
    Marquet, Pierre
    Kou, Shan Shan
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Date
    2017-04-14
    Permanent link to this record
    http://hdl.handle.net/10754/626506
    
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    Abstract
    Here we report a method for visualization of volumetric structural information of live biological samples with no exogenous contrast agents. The process is made possible through a technique that involves generation, synthesis and analysis of three-dimensional (3D) Fourier components of light diffracted by the sample. This leads to the direct recovery of quantitative cellular morphology with no iterative procedures for reduced computational complexity. Combing with the fact that the technique is easily adaptive to any imaging platform and requires minimum sample preparation, our proposed method is particularly promising for observing fast, volumetric and dynamic events previously only accessible through staining methods.
    Publisher
    arXiv
    arXiv
    1704.04445
    Additional Links
    http://arxiv.org/abs/1704.04445v1
    http://arxiv.org/pdf/1704.04445v1
    Collections
    Biological and Environmental Science and Engineering (BESE) Division; Preprints

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