Label-free three-dimensional (3D) structural imaging in live cells using intrinsic optical refractive index
Type
PreprintAuthors
Liu, ChenMalek, Michael
Poon, Ivan K. H.
Jiang, Lanzhou
Sheppard, Colin J. R.
Roberts, Ann
Quiney, Harry
Zhang, Douguo
Yuan, Xiaocong
Lin, Jiao
Depeursinge, Christian
Marquet, Pierre
Kou, Shan Shan
Date
2017-04-14Permanent link to this record
http://hdl.handle.net/10754/626506
Metadata
Show full item recordAbstract
Here we report a method for visualization of volumetric structural information of live biological samples with no exogenous contrast agents. The process is made possible through a technique that involves generation, synthesis and analysis of three-dimensional (3D) Fourier components of light diffracted by the sample. This leads to the direct recovery of quantitative cellular morphology with no iterative procedures for reduced computational complexity. Combing with the fact that the technique is easily adaptive to any imaging platform and requires minimum sample preparation, our proposed method is particularly promising for observing fast, volumetric and dynamic events previously only accessible through staining methods.Publisher
arXivarXiv
1704.04445Additional Links
http://arxiv.org/abs/1704.04445v1http://arxiv.org/pdf/1704.04445v1