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dc.contributor.authorPandit, Santosh
dc.contributor.authorRavikumar, Vaishnavi
dc.contributor.authorAbdel-Haleem, Alyaa M.
dc.contributor.authorDerouiche, Abderahmane
dc.contributor.authorMokkapati, V. R. S. S.
dc.contributor.authorSihlbom, Carina
dc.contributor.authorMineta, Katsuhiko
dc.contributor.authorGojobori, Takashi
dc.contributor.authorGao, Xin
dc.contributor.authorWesterlund, Fredrik
dc.contributor.authorMijakovic, Ivan
dc.date.accessioned2017-12-27T13:11:16Z
dc.date.available2017-12-27T13:11:16Z
dc.date.issued2017-12-22
dc.identifier.citationPandit S, Ravikumar V, Abdel-Haleem AM, Derouiche A, Mokkapati VRSS, et al. (2017) Low Concentrations of Vitamin C Reduce the Synthesis of Extracellular Polymers and Destabilize Bacterial Biofilms. Frontiers in Microbiology 8. Available: http://dx.doi.org/10.3389/fmicb.2017.02599.
dc.identifier.issn1664-302X
dc.identifier.pmid29317857
dc.identifier.doi10.3389/fmicb.2017.02599
dc.identifier.urihttp://hdl.handle.net/10754/626444
dc.description.abstractExtracellular polymeric substances (EPS) produced by bacteria form a matrix supporting the complex three-dimensional architecture of biofilms. This EPS matrix is primarily composed of polysaccharides, proteins and extracellular DNA. In addition to supporting the community structure, the EPS matrix protects bacterial biofilms from the environment. Specifically, it shields the bacterial cells inside the biofilm, by preventing antimicrobial agents from getting in contact with them, thereby reducing their killing effect. New strategies for disrupting the formation of the EPS matrix can therefore lead to a more efficient use of existing antimicrobials. Here we examined the mechanism of the known effect of vitamin C (sodium ascorbate) on enhancing the activity of various antibacterial agents. Our quantitative proteomics analysis shows that non-lethal concentrations of vitamin C inhibit bacterial quorum sensing and other regulatory mechanisms underpinning biofilm development. As a result, the EPS biosynthesis in reduced, and especially the polysaccharide component of the matrix is depleted. Once the EPS content is reduced beyond a critical point, bacterial cells get fully exposed to the medium. At this stage, the cells are more susceptible to killing, either by vitamin C-induced oxidative stress as reported here, or by other antimicrobials or treatments.
dc.description.sponsorshipThis work was funded by grants from the Chalmers University of Technology and VINNOVA to IM and FW, and ÅForsk to IM.
dc.publisherFrontiers Media SA
dc.relation.urlhttps://www.frontiersin.org/articles/10.3389/fmicb.2017.02599/full
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleLow Concentrations of Vitamin C Reduce the Synthesis of Extracellular Polymers and Destabilize Bacterial Biofilms
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer Science Program
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.identifier.journalFrontiers in Microbiology
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionSystems and Synthetic Biology Division, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden
dc.contributor.institutionProteomics Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
dc.contributor.institutionNovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark
kaust.personAbdel-Haleem, Alyaa M.
kaust.personMineta, Katsuhiko
kaust.personGojobori, Takashi
kaust.personGao, Xin
refterms.dateFOA2018-06-14T02:15:01Z


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This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.