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dc.contributor.authorTashkandi, Manal
dc.contributor.authorAli, Zahir
dc.contributor.authorAljedaani, Fatimah R.
dc.contributor.authorShami, Ashwag
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2019-03-17T12:21:27Z
dc.date.available2017-12-27T13:11:15Z
dc.date.available2019-03-17T12:21:27Z
dc.date.issued2018-10-05
dc.identifier.citationTashkandi M, Ali Z, Aljedaani F, Shami A, Mahfouz MM (2018) Engineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato. Plant Signaling & Behavior 13: e1525996. Available: http://dx.doi.org/10.1080/15592324.2018.1525996.
dc.identifier.issn1559-2324
dc.identifier.doi10.1080/15592324.2018.1525996
dc.identifier.urihttp://hdl.handle.net/10754/626438
dc.description.abstractCRISPR/Cas systems confer molecular immunity against phages and conjugative plasmids in prokaryotes. Recently, CRISPR/Cas9 systems have been used to confer interference against eukaryotic viruses. Here, we engineered Nicotiana benthamiana and tomato (Solanum lycopersicum) plants with the CRISPR/Cas9 system to confer immunity against the Tomato yellow leaf curl virus (TYLCV). Targeting the TYLCV genome with Cas9-single guide RNA at the sequences encoding the coat protein (CP) or replicase (Rep) resulted in efficient virus interference, as evidenced by low accumulation of the TYLCV DNA genome in the transgenic plants. The CRISPR/Cas9-based immunity remained active across multiple generations in the N. benthamiana and tomato plants. Together, our results confirmed the efficiency of the CRISPR/Cas9 system for stable engineering of TYLCV resistance in N. benthamiana and tomato, and opens the possibilities of engineering virus resistance against single and multiple infectious viruses in other crops.
dc.description.sponsorshipWe thank the members of the Laboratory for Genome Engineering for their continuous constructive discussions and comments on the manuscript. This publication is based upon work supported by the King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) under Award No. OSR-2015-CRG4-2647.
dc.publisherInforma UK Limited
dc.relation.urlhttps://www.tandfonline.com/doi/full/10.1080/15592324.2018.1525996
dc.rightsArchived with thanks to Plant Signaling & Behavior
dc.subjectVirus resistance
dc.subjectTomato (Solanum Lycopersicum)
dc.subjectCrispr/cas9 System
dc.subjectTomato Yellow Leaf Curl Virus (Tylcv)
dc.subjectMolecular Immunity
dc.subjectTomato Transformation And Regeneration
dc.titleEngineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentLaboratory for Genome Engineering
dc.contributor.departmentPlant Science
dc.identifier.journalPlant Signaling & Behavior
dc.eprint.versionPost-print
dc.contributor.institutionCollege of Science, Biology Department, Kingdom of Saudi Arabia (KSA), Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia
kaust.personTashkandi, Manal
kaust.personAli, Zahir
kaust.personAljedaani, Fatimah
kaust.personShami, Ashwag
kaust.personMahfouz, Magdy M.
kaust.grant.numberOSR-2015-CRG4-2647
dc.relation.issupplementedbyDOI:10.6084/m9.figshare.7172468
refterms.dateFOA2018-06-14T00:05:08Z
display.relations<b>Is Supplemented By:</b><br/> <ul><li><i>[Dataset]</i> <br/> Tashkandi, M., Ali, Z., Aljedaani, F., Ashwag Shami, &amp; Magdy M. Mahfouz. (2018). <i>Engineering resistance against <i>Tomato yellow leaf curl virus</i> via the CRISPR/Cas9 system in tomato</i> [Data set]. Taylor &amp; Francis. https://doi.org/10.6084/M9.FIGSHARE.7172468. DOI: <a href="https://doi.org/10.6084/m9.figshare.7172468" >10.6084/m9.figshare.7172468</a> Handle: <a href="http://hdl.handle.net/10754/668116" >10754/668116</a></a></li></ul>
dc.date.published-online2018-10-05
dc.date.published-print2018-10-03


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