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dc.contributor.authorMahfouz, Magdy M.
dc.contributor.authorTashkandi, Manal
dc.contributor.authorAli, Zahir
dc.contributor.authorAljedaani, Fatimah R.
dc.contributor.authorShami, Ashwag
dc.date.accessioned2017-12-27T13:11:15Z
dc.date.available2017-12-27T13:11:15Z
dc.date.issued2017-12-22
dc.identifier.citationMahfouz M, Tashkandi M, ALI Z, Aljedaani F, Shami A (2017) Engineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato. Available: http://dx.doi.org/10.1101/237735.
dc.identifier.doi10.1101/237735
dc.identifier.urihttp://hdl.handle.net/10754/626438.1
dc.description.abstractCRISPR/Cas systems confer molecular immunity against phages and conjugative plasmids in prokaryotes. Recently, CRISPR/Cas9 systems have been used to confer interference against eukaryotic viruses. Here, we engineered Nicotiana benthamiana and tomato (Solanum lycopersicum) plants with the CRISPR/Cas9 system to confer immunity against the Tomato yellow leaf curl virus (TYLCV). Targeting the TYLCV genome with Cas9-single guide RNA at the sequences encoding the coat protein (CP) or replicase (Rep) resulted in efficient virus interference, as evidenced by low accumulation of the TYLCV DNA genome in the transgenic plants. The CRISPR/Cas9-based immunity remained active across multiple generations in the N. benthamiana and tomato plants. Together, our results confirmed the efficiency of the CRISPR/Cas9 system for stable engineering of TYLCV resistance in N. benthamiana and tomato, and opens the possibilities of engineering virus resistance against single and multiple infectious viruses in other crops.
dc.description.sponsorshipWe thank the members of the Laboratory for Genome Engineering for their continuous constructive discussions and comments on the manuscript. This publication is based upon work supported by the King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) under Award No. OSR-2015-CRG4-2647.
dc.publisherCold Spring Harbor Laboratory
dc.relation.urlhttps://www.biorxiv.org/content/early/2017/12/21/237735
dc.rightsArchived with thanks to bioRxiv
dc.subjectCRISPR/Cas9 system
dc.subjectTomato yellow leaf curl virus (TYLCV)
dc.subjectvirus resistance
dc.subjectmolecular immunity
dc.subjecttomato (Solanum lycopersicum)
dc.subjecttomato transformation and regeneration
dc.titleEngineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato
dc.typePreprint
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentPlant Science Program
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentBioscience Program
dc.contributor.departmentLaboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia
dc.eprint.versionPre-print
kaust.personMahfouz, Magdy M.
kaust.personTashkandi, Manal
kaust.personAli, Zahir
kaust.personAljedaani, Fatimah R.
kaust.personShami, Ashwag
kaust.grant.numberOSR-2015-CRG4-2647
refterms.dateFOA2018-06-14T00:05:08Z


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