Endosomal Escape and Delivery of CRISPR/Cas9 Genome Editing Machinery Enabled by Nanoscale Zeolitic Imidazolate Framework
Type
ArticleAuthors
Alsaiari, Shahad K.
Patil, Sachin

Alyami, Mram Z.

Alamoudi, Kholod

Aleisa, Fajr A

Merzaban, Jasmeen

Li, Mo

Khashab, Niveen M.

KAUST Department
Advanced Membranes and Porous Materials Research CenterBiological and Environmental Sciences and Engineering (BESE) Division
Bioscience Program
Chemical Science Program
Physical Science and Engineering (PSE) Division
Smart Hybrid Materials (SHMs) lab
Date
2017-12-27Online Publication Date
2017-12-27Print Publication Date
2018-01-10Permanent link to this record
http://hdl.handle.net/10754/626437
Metadata
Show full item recordAbstract
CRISPR/Cas9 is a combined protein (Cas9) and an engineered single guide RNA (sgRNA) genome editing platform that offers revolutionary solutions to genetic diseases. It has, however, a double delivery problem owning to the large protein size and the highly charged RNA component. In this work, we report the first example of CRISPR/Cas9 encapsulated by nanoscale zeolitic imidazole frameworks (ZIFs) with a loading efficiency of 17% and enhanced endosomal escape promoted by the protonated imidazole moieties. The gene editing potential of CRISPR/Cas9 encapsulated by ZIF-8 (CC-ZIFs) is further verified by knocking down the gene expression of green fluorescent protein by 37% over 4 days employing CRISPR/Cas9 machinery. The nanoscale CC-ZIFs are biocompatible and easily scaled-up offering excellent loading capacity and controlled co-delivery of intact Cas9 protein and sgRNA.Citation
Alsaiari SK, Patil S, Alyami M, Alamoudi K, Aleisa fajr, et al. (2017) Endosomal Escape and Delivery of CRISPR/Cas9 Genome Editing Machinery Enabled by Nanoscale Zeolitic Imidazolate Framework. Journal of the American Chemical Society. Available: http://dx.doi.org/10.1021/jacs.7b11754.Publisher
American Chemical Society (ACS)PubMed ID
29272114Additional Links
http://pubs.acs.org/doi/10.1021/jacs.7b11754ae974a485f413a2113503eed53cd6c53
10.1021/jacs.7b11754
Scopus Count
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