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dc.contributor.authorBenavente, Ernest Diez
dc.contributor.authorde Sessions, Paola Florez
dc.contributor.authorMoon, Robert W.
dc.contributor.authorGrainger, Munira
dc.contributor.authorHolder, Anthony A.
dc.contributor.authorBlackman, Michael J.
dc.contributor.authorRoper, Cally
dc.contributor.authorDrakeley, Christopher J.
dc.contributor.authorPain, Arnab
dc.contributor.authorSutherland, Colin J.
dc.contributor.authorHibberd, Martin L.
dc.contributor.authorCampino, Susana
dc.contributor.authorClark, Taane G.
dc.date.accessioned2017-12-21T13:57:03Z
dc.date.available2017-12-21T13:57:03Z
dc.date.issued2017-12-16
dc.identifier.citationBenavente ED, de Sessions PF, Moon RW, Grainger M, Holder AA, et al. (2017) A reference genome and methylome for the Plasmodium knowlesi A1-H.1 line. International Journal for Parasitology. Available: http://dx.doi.org/10.1016/j.ijpara.2017.09.008.
dc.identifier.issn0020-7519
dc.identifier.pmid29258833
dc.identifier.doi10.1016/j.ijpara.2017.09.008
dc.identifier.urihttp://hdl.handle.net/10754/626402
dc.description.abstractPlasmodium knowlesi, a common parasite of macaques, is recognized as a significant cause of human malaria in Malaysia. The P. knowlesi A1H1 line has been adapted to continuous culture in human erythrocytes, successfully providing an in vitro model to study the parasite. We have assembled a reference genome for the PkA1-H.1 line using PacBio long read combined with Illumina short read sequence data. Compared with the H-strain reference, the new reference has improved genome coverage and a novel description of methylation sites. The PkA1-H.1 reference will enhance the capabilities of the in vitro model to improve the understanding of P. knowlesi infection in humans.
dc.description.sponsorshipTGC is funded by the Medical Research Council (MRC), UK (Grant no. MR/K000551/1, MR/M01360X/1, MR/N010469/1). SC and CR are funded by the MRC (Grant no. MR/M01360X/1). RWM is supported by an MRC Career Development Award jointly funded by the MRC and UK Department for International Development. This work was supported in part by the Francis Crick Institute, UK which receives its core funding from Cancer Research, UK (FC001097, FC001043), the MRC (FC001097, FC001043), and the Wellcome Trust, UK (FC001097, FC001043) We gratefully acknowledge the Scientific Computing Group for data management and computer infrastructure at Genome Institute of Singapore for their help. Bioinformatic analysis was performed on the MRC, UK, funded eMedLab computing resource.
dc.publisherElsevier BV
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0020751917303466
dc.rights© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectPlasmodium knowlesi
dc.subjectReference genome
dc.subjectPkA1-H.1
dc.subjectSequencing
dc.subjectMalaria
dc.subjectMethylation
dc.titleA reference genome and methylome for the Plasmodium knowlesi A1-H.1 line
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.identifier.journalInternational Journal for Parasitology
dc.eprint.versionPost-print
dc.contributor.institutionFaculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
dc.contributor.institutionGenomics Institute Singapore, Singapore
dc.contributor.institutionThe Francis Crick Institute, 1 Midland Road, London NW1 1AT, United Kingdom
dc.contributor.institutionFaculty of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, London, United Kingdom
kaust.personPain, Arnab
refterms.dateFOA2018-06-13T19:09:54Z
dc.date.published-online2017-12-16
dc.date.published-print2018-03


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© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Except where otherwise noted, this item's license is described as © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/