Spliceosomal protein U1A is involved in alternative splicing and salt stress tolerance in Arabidopsis thaliana
KAUST Grant NumberBAS/1/1007-01-01
MetadataShow full item record
AbstractSoil salinity is a significant threat to sustainable agricultural production worldwide. Plants must adjust their developmental and physiological processes to cope with salt stress. Although the capacity for adaptation ultimately depends on the genome, the exceptional versatility in gene regulation provided by the spliceosome-mediated alternative splicing (AS) is essential in these adaptive processes. However, the functions of the spliceosome in plant stress responses are poorly understood. Here, we report the in-depth characterization of a U1 spliceosomal protein, AtU1A, in controlling AS of pre-mRNAs under salt stress and salt stress tolerance in Arabidopsis thaliana. The atu1a mutant was hypersensitive to salt stress and accumulated more reactive oxygen species (ROS) than the wild-type under salt stress. RNA-seq analysis revealed that AtU1A regulates AS of many genes, presumably through modulating recognition of 5′ splice sites. We showed that AtU1A is associated with the pre-mRNA of the ROS detoxification-related gene ACO1 and is necessary for the regulation of ACO1 AS. ACO1 is important for salt tolerance because ectopic expression of ACO1 in the atu1a mutant can partially rescue its salt hypersensitive phenotype. Our findings highlight the critical role of AtU1A as a regulator of pre-mRNA processing and salt tolerance in plants.
CitationGu J, Xia Z, Luo Y, Jiang X, Qian B, et al. (2017) Spliceosomal protein U1A is involved in alternative splicing and salt stress tolerance in Arabidopsis thaliana. Nucleic Acids Research. Available: http://dx.doi.org/10.1093/nar/gkx1229.
SponsorsWe acknowledge the ABRC for providing the T-DNA insertion lines. We thank Prof. Marvin Wickens (University of Wisconsin-Madison) for providing the yeast three-hybrid system. National Natural Science Foundation of China [31670250 to Z.W.]; Natural Science Foundation of Hainan Province [20163041 to Z.W.]; Hainan University Startup Fund [KYQD1562 to Z.W.]; YNTC [YNTC-2016YN22 to H.X.]; KAUST Faculty Baseline Funds [#BAS/1/1007-01-01 to L.M.X.]; National Key Technology Support Program [2015BAD01B02 to Y.H.L., X.Y.J.]; National Science Foundation [MCB0950242 to J.H.Z.]. Funding for open access charge: Hainan University Startup Fund [KYQD1562].
PublisherOxford University Press (OUP)
JournalNucleic Acids Research
Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact firstname.lastname@example.org
- SKIP Confers Osmotic Tolerance during Salt Stress by Controlling Alternative Gene Splicing in Arabidopsis.
- Authors: Feng J, Li J, Gao Z, Lu Y, Yu J, Zheng Q, Yan S, Zhang W, He H, Ma L, Zhu Z
- Issue date: 2015 Jul
- Genome-wide analysis of alternative splicing of pre-mRNA under salt stress in Arabidopsis.
- Authors: Ding F, Cui P, Wang Z, Zhang S, Ali S, Xiong L
- Issue date: 2014 Jun 4
- ITN1, a novel gene encoding an ankyrin-repeat protein that affects the ABA-mediated production of reactive oxygen species and is involved in salt-stress tolerance in Arabidopsis thaliana.
- Authors: Sakamoto H, Matsuda O, Iba K
- Issue date: 2008 Nov
- Nuclear-localized AtHSPR links abscisic acid-dependent salt tolerance and antioxidant defense in Arabidopsis.
- Authors: Yang T, Zhang L, Hao H, Zhang P, Zhu H, Cheng W, Wang Y, Wang X, Wang C
- Issue date: 2015 Dec
- A DEAD box RNA helicase is critical for pre-mRNA splicing, cold-responsive gene regulation, and cold tolerance in Arabidopsis.
- Authors: Guan Q, Wu J, Zhang Y, Jiang C, Liu R, Chai C, Zhu J
- Issue date: 2013 Jan