Evidence for miRNA-mediated modulation of the host transcriptome in cnidarian-dinoflagellate symbiosis
Name:
Baumgarten_et_al-2017-Molecular_Ecology.pdf
Size:
1.276Mb
Format:
PDF
Description:
Accepted Manuscript
Name:
4fcd65ca019287d8ad78b08baf5224021820cdff22aea57c9cced41a4c4354f8.sup-1.pdf
Size:
88.37Kb
Format:
PDF
Description:
Supplemental files
Name:
4fcd65ca019287d8ad78b08baf5224021820cdff22aea57c9cced41a4c4354f8.sup-2.pdf
Size:
82.17Kb
Format:
PDF
Description:
Supplemental files
Name:
4fcd65ca019287d8ad78b08baf5224021820cdff22aea57c9cced41a4c4354f8.sup-3.pdf
Size:
221.4Kb
Format:
PDF
Description:
Supplemental files
Name:
mec14452-sup-0004-TableS1-4.xlsx
Size:
547.0Kb
Format:
Microsoft Excel 2007
Description:
Supplemental files
Type
ArticleAuthors
Baumgarten, Sebastian
Cziesielski, Maha Joana

Thomas, Ludivine
Michell, Craig
Esherick, Lisl Y.
Pringle, John R.
Aranda, Manuel

Voolstra, Christian R.

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Core Lab
Computational Bioscience Research Center (CBRC)
Marine Science Program
Red Sea Research Center (RSRC)
Date
2017-12-23Online Publication Date
2017-12-23Print Publication Date
2018-01Permanent link to this record
http://hdl.handle.net/10754/626371
Metadata
Show full item recordAbstract
Reef-building corals and other cnidarians living in symbiotic relationships with intracellular, photosynthetic dinoflagellates in the genus Symbiodinium undergo transcriptomic changes during infection with the algae and maintenance of the endosymbiont population. However, the precise regulatory mechanisms modulating the host transcriptome are unknown. Here we report apparent post-transcriptional gene regulation by miRNAs in the sea anemone Aiptasia, a model system for cnidarian-dinoflagellate endosymbiosis. Aiptasia encodes mainly species-specific miRNAs, and there appears to have been recent differentiation within the Aiptasia genome of miRNAs that are commonly conserved among anthozoan cnidarians. Analysis of miRNA expression showed that both conserved and species-specific miRNAs are differentially expressed in response to endosymbiont infection. Using cross-linking immunoprecipitation of Argonaute, the central protein of the miRNA-induced silencing complex, we identified miRNA binding sites on a transcriptome-wide scale and found that the targets of the miRNAs regulated in response to symbiosis include genes previously implicated in biological processes related to Symbiodinium infection. Our study shows that cnidarian miRNAs recognize their mRNA targets via high-complementarity target binding and suggests that miRNA-mediated modulations of genes and pathways are important during the onset and maintenance of cnidarian-dinoflagellate endosymbiosis. This article is protected by copyright. All rights reserved.Citation
Baumgarten S, Cziesielski MJ, Thomas L, Michell CT, Esherick LY, et al. (2017) Evidence for miRNA-mediated modulation of the host transcriptome in cnidarian-dinoflagellate symbiosis. Molecular Ecology. Available: http://dx.doi.org/10.1111/mec.14452.Sponsors
Research reported in this publication was supported by the King Abdullah University of Science and Technology (KAUST) and by Gordon and Betty Moore Foundation Grant 2629.01 (to J.R.P.). We thank the Bioscience Core lab and the Imaging and Characterization Core lab at KAUST for help with the protein characterization and microscopy. Figure 5 was created by Heno Hwang, scientific illustrator at KAUST.Publisher
WileyJournal
Molecular EcologyPubMed ID
29218749Additional Links
http://onlinelibrary.wiley.com/doi/10.1111/mec.14452/abstractae974a485f413a2113503eed53cd6c53
10.1111/mec.14452
Scopus Count
Related articles
- Genome-wide polymorphism and signatures of selection in the symbiotic sea anemone Aiptasia.
- Authors: Bellis ES, Howe DK, Denver DR
- Issue date: 2016 Feb 29
- Development and Symbiosis Establishment in the Cnidarian Endosymbiosis Model Aiptasia sp.
- Authors: Bucher M, Wolfowicz I, Voss PA, Hambleton EA, Guse A
- Issue date: 2016 Jan 25
- Optimal nutrient exchange and immune responses operate in partner specificity in the cnidarian-dinoflagellate symbiosis.
- Authors: Matthews JL, Crowder CM, Oakley CA, Lutz A, Roessner U, Meyer E, Grossman AR, Weis VM, Davy SK
- Issue date: 2017 Dec 12
- Generation and analysis of transcriptomic resources for a model system on the rise: the sea anemone Aiptasia pallida and its dinoflagellate endosymbiont.
- Authors: Sunagawa S, Wilson EC, Thaler M, Smith ML, Caruso C, Pringle JR, Weis VM, Medina M, Schwarz JA
- Issue date: 2009 Jun 5
- Transcriptome analysis of a cnidarian-dinoflagellate mutualism reveals complex modulation of host gene expression.
- Authors: Rodriguez-Lanetty M, Phillips WS, Weis VM
- Issue date: 2006 Feb 10