• Login
    View Item 
    •   Home
    • Research
    • Articles
    • View Item
    •   Home
    • Research
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguideTheses and Dissertations LibguideSubmit an Item

    Statistics

    Display statistics

    Evidence for miRNA-mediated modulation of the host transcriptome in cnidarian-dinoflagellate symbiosis

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Baumgarten_et_al-2017-Molecular_Ecology.pdf
    Size:
    1.276Mb
    Format:
    PDF
    Description:
    Accepted Manuscript
    Download
    Thumbnail
    Name:
    4fcd65ca019287d8ad78b08baf5224021820cdff22aea57c9cced41a4c4354f8.sup-1.pdf
    Size:
    88.37Kb
    Format:
    PDF
    Description:
    Supplemental files
    Download
    Thumbnail
    Name:
    4fcd65ca019287d8ad78b08baf5224021820cdff22aea57c9cced41a4c4354f8.sup-2.pdf
    Size:
    82.17Kb
    Format:
    PDF
    Description:
    Supplemental files
    Download
    Thumbnail
    Name:
    4fcd65ca019287d8ad78b08baf5224021820cdff22aea57c9cced41a4c4354f8.sup-3.pdf
    Size:
    221.4Kb
    Format:
    PDF
    Description:
    Supplemental files
    Download
    Thumbnail
    Name:
    mec14452-sup-0004-TableS1-4.xlsx
    Size:
    547.0Kb
    Format:
    Microsoft Excel 2007
    Description:
    Supplemental files
    Download
    View more filesView fewer files
    Type
    Article
    Authors
    Baumgarten, Sebastian cc
    Cziesielski, Maha Joana cc
    Thomas, Ludivine
    Michell, Craig
    Esherick, Lisl Y.
    Pringle, John R.
    Aranda, Manuel cc
    Voolstra, Christian R. cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Core Lab
    Computational Bioscience Research Center (CBRC)
    Marine Science Program
    Red Sea Research Center (RSRC)
    Date
    2017-12-23
    Online Publication Date
    2017-12-23
    Print Publication Date
    2018-01
    Permanent link to this record
    http://hdl.handle.net/10754/626371
    
    Metadata
    Show full item record
    Abstract
    Reef-building corals and other cnidarians living in symbiotic relationships with intracellular, photosynthetic dinoflagellates in the genus Symbiodinium undergo transcriptomic changes during infection with the algae and maintenance of the endosymbiont population. However, the precise regulatory mechanisms modulating the host transcriptome are unknown. Here we report apparent post-transcriptional gene regulation by miRNAs in the sea anemone Aiptasia, a model system for cnidarian-dinoflagellate endosymbiosis. Aiptasia encodes mainly species-specific miRNAs, and there appears to have been recent differentiation within the Aiptasia genome of miRNAs that are commonly conserved among anthozoan cnidarians. Analysis of miRNA expression showed that both conserved and species-specific miRNAs are differentially expressed in response to endosymbiont infection. Using cross-linking immunoprecipitation of Argonaute, the central protein of the miRNA-induced silencing complex, we identified miRNA binding sites on a transcriptome-wide scale and found that the targets of the miRNAs regulated in response to symbiosis include genes previously implicated in biological processes related to Symbiodinium infection. Our study shows that cnidarian miRNAs recognize their mRNA targets via high-complementarity target binding and suggests that miRNA-mediated modulations of genes and pathways are important during the onset and maintenance of cnidarian-dinoflagellate endosymbiosis. This article is protected by copyright. All rights reserved.
    Citation
    Baumgarten S, Cziesielski MJ, Thomas L, Michell CT, Esherick LY, et al. (2017) Evidence for miRNA-mediated modulation of the host transcriptome in cnidarian-dinoflagellate symbiosis. Molecular Ecology. Available: http://dx.doi.org/10.1111/mec.14452.
    Sponsors
    Research reported in this publication was supported by the King Abdullah University of Science and Technology (KAUST) and by Gordon and Betty Moore Foundation Grant 2629.01 (to J.R.P.). We thank the Bioscience Core lab and the Imaging and Characterization Core lab at KAUST for help with the protein characterization and microscopy. Figure 5 was created by Heno Hwang, scientific illustrator at KAUST.
    Publisher
    Wiley
    Journal
    Molecular Ecology
    DOI
    10.1111/mec.14452
    PubMed ID
    29218749
    Additional Links
    http://onlinelibrary.wiley.com/doi/10.1111/mec.14452/abstract
    ae974a485f413a2113503eed53cd6c53
    10.1111/mec.14452
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Red Sea Research Center (RSRC); Marine Science Program; Computational Bioscience Research Center (CBRC); Bioscience Core Lab; Reef Genomics, part of the Global Ocean Genome Project

    entitlement

    Related articles

    • Genome-wide polymorphism and signatures of selection in the symbiotic sea anemone Aiptasia.
    • Authors: Bellis ES, Howe DK, Denver DR
    • Issue date: 2016 Feb 29
    • Development and Symbiosis Establishment in the Cnidarian Endosymbiosis Model Aiptasia sp.
    • Authors: Bucher M, Wolfowicz I, Voss PA, Hambleton EA, Guse A
    • Issue date: 2016 Jan 25
    • Optimal nutrient exchange and immune responses operate in partner specificity in the cnidarian-dinoflagellate symbiosis.
    • Authors: Matthews JL, Crowder CM, Oakley CA, Lutz A, Roessner U, Meyer E, Grossman AR, Weis VM, Davy SK
    • Issue date: 2017 Dec 12
    • Generation and analysis of transcriptomic resources for a model system on the rise: the sea anemone Aiptasia pallida and its dinoflagellate endosymbiont.
    • Authors: Sunagawa S, Wilson EC, Thaler M, Smith ML, Caruso C, Pringle JR, Weis VM, Medina M, Schwarz JA
    • Issue date: 2009 Jun 5
    • Transcriptome analysis of a cnidarian-dinoflagellate mutualism reveals complex modulation of host gene expression.
    • Authors: Rodriguez-Lanetty M, Phillips WS, Weis VM
    • Issue date: 2006 Feb 10
    DSpace software copyright © 2002-2023  DuraSpace
    Quick Guide | Contact Us | KAUST University Library
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.