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    Membrane-Mimic Nanoparticles for Drug and Gene Delivery

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    Name:
    Kholod Alamoudi Dissertation.pdf
    Size:
    24.80Mb
    Format:
    PDF
    Description:
    Kholod Alamoudi Dissertation
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    Type
    Dissertation
    Authors
    Alamoudi, Kholod cc
    Advisors
    Khashab, Niveen M. cc
    Committee members
    Saikaly, Pascal cc
    Merzaban, Jasmeen cc
    Tamanoi, Fuyu
    Program
    Bioscience
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Date
    2017-12
    Embargo End Date
    2018-12-06
    Permanent link to this record
    http://hdl.handle.net/10754/626304
    
    Metadata
    Show full item record
    Access Restrictions
    At the time of archiving, the student author of this dissertation opted to temporarily restrict access to it. The full text of this dissertation became available to the public after the expiration of the embargo on 2018-12-06.
    Abstract
    Nanoscale organic particles have gained a prominent role in drug and gene delivery field. As the nature of the nanoparticle’s (NPs) surface plays a major role in their targeting efficiency, bioavailability, and cytotoxicity, membrane-mimic nanoparticles are considered highly attractive materials for in vivo and in vitro applications. Synthetic membrane vesicles (liposomes) and nanoconstructs built with native cancer cellular membrane are excellent scaffolds to improve cellular delivery. Liposomes have been extensively used due to their high loading capacity, biocompatibility and biodegradability. However, modifications with stimuli responsive materials are highly needed to improve their stability and turn them active participants in controlled delivery. Towards a nature inspired approach, reconstructed bilayers from cell membrane are a good candidate to enhance NP’s targeting ability and biocompatibility. The primary focus of this research is to develop smart responsive (lipid) membrane coated NPs with surface modifications for controlled and targeted drug and/or gene delivery for application in cancer therapy. Three approaches have been developed, namely i) liposomes as thermoresponsive nanocarriers for the delivery of genetic material; ii) magnetically photosensitive liposome hybrids and iii) biomimetic periodic mesoporous organo silica engineered for better a biocompatibility and targeting capabilities. In the first project synthetic liposomes were loaded with ammonium bicarbonate salt (ABC) and siRNA. The combination of lipids chosen and the relative ratios allowed the rapid release of the genetic material to the multi drug resistant cancer cells studied, upon external heat trigger. This design has improved the gene silencing efficiency via successful endosomal escape. In the second project, SPIO@Au nanoparticles were imbedded in the lipid bilayer to produce a photo/thermal responsive carrier that could be also used in cell imaging besides gene transfection and drug delivery. For the final project, a nature inspired coating was used in periodic mesoporous organosilica (PMO) NPs. PMOs were functionalized with colorectal cancer cell membrane. The resulting CC@NH2-TSPMOs, holding the diverse cancer cell membrane antigens showed a promising potential towards disease targeting and improved pharmacokinetics. This research confirms the notion of how nanotechnology engineering approaches are effective to improve the quality and effectiveness of cancer therapeutics.
    Citation
    Alamoudi, K. (2017). Membrane-Mimic Nanoparticles for Drug and Gene Delivery. KAUST Research Repository. https://doi.org/10.25781/KAUST-79185
    DOI
    10.25781/KAUST-79185
    ae974a485f413a2113503eed53cd6c53
    10.25781/KAUST-79185
    Scopus Count
    Collections
    Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; PhD Dissertations

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