Show simple item record

dc.contributor.authorMarini, Monica
dc.contributor.authorAllione, Marco
dc.contributor.authorLopatin, Sergei
dc.contributor.authorMoretti, Manola
dc.contributor.authorGiugni, Andrea
dc.contributor.authorTorre, Bruno
dc.contributor.authorDi Fabrizio, Enzo M.
dc.date.accessioned2017-12-05T06:12:00Z
dc.date.available2017-12-05T06:12:00Z
dc.date.issued2017-12-01
dc.identifier.citationMarini M, Allione M, Lopatin S, Moretti M, Giugni A, et al. (2017) Suspended DNA structural characterization by TEM diffraction. Microelectronic Engineering. Available: http://dx.doi.org/10.1016/j.mee.2017.11.020.
dc.identifier.issn0167-9317
dc.identifier.doi10.1016/j.mee.2017.11.020
dc.identifier.urihttp://hdl.handle.net/10754/626286
dc.description.abstractIn this work, micro-fabrication, super-hydrophobic properties and a physiologically compatible preparation step are combined and tailored to obtain background free biological samples to be investigated by Transmission Electron Microscopy (TEM) diffraction technique. The validation was performed evaluating a well-known parameter such as the DNA interbases value. The diffraction spacing measured is in good agreement with those obtained by HRTEM direct metrology and by traditional X-Ray diffraction. This approach addresses single molecule studies in a simplified and reproducible straightforward way with respect to more conventional and widely used techniques. In addition, it overcomes the need of long and elaborated samples preparations: the sample is in its physiological environment and the HRTEM data acquisition occurs without any background interference, coating, staining or additional manipulation. The congruence in the results reported in this paper makes the application of this approach extremely promising towards those molecules for which crystallization remains a hurdle, such as cell membrane proteins and fibrillar proteins.
dc.description.sponsorshipThe authors acknowledge financial support from the KAUST start-up funding and from Italian Ministry of Health under the projects: Project no.: GR-2010-2320665 and Project no.: GR-2010-2311677.
dc.publisherElsevier BV
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0167931717303969
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Microelectronic Engineering. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Microelectronic Engineering, [, , (2017-12-01)] DOI: 10.1016/j.mee.2017.11.020 . © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectDNA
dc.subjectSuper-hydrophobic devices
dc.subjectDiffraction
dc.subjectInterbases distance
dc.titleSuspended DNA structural characterization by TEM diffraction
dc.typeArticle
dc.contributor.departmentElectron Microscopy
dc.contributor.departmentImaging and Characterization Core Lab
dc.contributor.departmentMaterial Science and Engineering Program
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalMicroelectronic Engineering
dc.eprint.versionPost-print
kaust.personMarini, Monica
kaust.personAllione, Marco
kaust.personLopatin, Sergei
kaust.personMoretti, Manola
kaust.personGiugni, Andrea
kaust.personTorre, Bruno
kaust.personDi Fabrizio, Enzo M.
dc.date.published-online2017-12-01
dc.date.published-print2018-02


Files in this item

Thumbnail
Name:
1-s2.0-S0167931717303969-main.pdf
Size:
1009.Kb
Format:
PDF
Description:
Accepted Manuscript
Thumbnail
Name:
1-s2.0-S0167931717303969-fx1.jpg
Size:
33.89Kb
Format:
JPEG image
Description:
Graphical abstract

This item appears in the following Collection(s)

Show simple item record